Document Detail


Effects of 1,25-dihydroxyvitamin D3 on calcium and phosphorus homeostasis in sheep fed diets either adequate or restricted in calcium content.
MedLine Citation:
PMID:  19962267     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
It was the aim of the present study to collect basic data on calcium (Ca) and phosphorus (P) homoeostasis in sheep. Two series of experiments were carried out to investigate the effects of 1,25-dihydroxyvitammin D(3) (calcitriol) in supraphysiological dosage in combination with varying alimentary Ca supply. In the first series, blood samples were collected over 72 h to determine the concentrations of total Ca (Ca), ionized Ca (Ca(2+)), inorganic phosphate (P(i)), and the bone resorption marker CrossLaps (CL). In the second series, measurements were carried out over 12h. In addition, urine samples were collected to calculate the fractional excretions (FE) of Ca and P(i). Changes in plasma macromineral concentrations (P<0.01) as well as in CL (P<0.001) and endogenous calcitriol (P<0.05) were observed in the alimentary Ca-restricted animals, indicating that the reduction of daily Ca intake challenged the animals' macromineral homeostatic mechanisms. However, the Ca-restricted diet had an effect on neither FE of Ca nor on FE of P(i). The treatment resulted in peak serum calcitriol concentrations between 1,900 and 2,500 pg/mL, and supraphysiological concentrations were maintained for the next 48 h. Irrespective of dietary Ca, calcitriol had hypercalcemic and hyperphosphatemic effects. An increase in CL was revealed only in the Ca-restricted, calcitriol-treated sheep (P<0.01), reflecting a remarkable enhancement of Ca mobilization from the bone by calcitriol exclusively in this group. From these data, it can be concluded that the sheep can be a suitable animal model for studying catabolic effects of Ca deficiency and calcitriol on bone metabolism.
Authors:
M R Wilkens; N Mrochen; G Breves; B Schröder
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-11-25
Journal Detail:
Title:  Domestic animal endocrinology     Volume:  38     ISSN:  1879-0054     ISO Abbreviation:  Domest. Anim. Endocrinol.     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-03-02     Completed Date:  2010-08-09     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8505191     Medline TA:  Domest Anim Endocrinol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  190-9     Citation Subset:  IM    
Copyright Information:
Copyright 2009 Elsevier Inc. All rights reserved.
Affiliation:
Department of Physiology, University of Veterinary Medicine Hannover, Bischofsholer Damm 15, 30173 Hannover, Germany. Mirja.Wilkens@tiho-hannover.de
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MeSH Terms
Descriptor/Qualifier:
Animal Feed*
Animals
Biological Markers / metabolism
Bone Resorption / metabolism
Bone and Bones / metabolism
Calcitriol / administration & dosage*,  metabolism
Calcium / metabolism*
Calcium, Dietary / administration & dosage
Collagen / metabolism*
Female
Hypercalcemia / chemically induced
Hyperphosphatemia / chemically induced
Peptide Fragments / metabolism*
Phosphates / metabolism
Phosphorus / metabolism*
Sheep, Domestic
Chemical
Reg. No./Substance:
0/Biological Markers; 0/Calcium, Dietary; 0/Peptide Fragments; 0/Phosphates; 0/glutamyl-lysyl-alanyl-histidyl-aspartyl-glycyl-glycyl-arginine; 32222-06-3/Calcitriol; 7440-70-2/Calcium; 7723-14-0/Phosphorus; 9007-34-5/Collagen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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