Document Detail


Effectiveness of selective genotyping for detection of quantitative trait loci: an analysis of grain and malt quality traits in three barley populations.
MedLine Citation:
PMID:  12502257     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Marker genotype data and grain and malt quality phenotype data from three barley (Hordeum vulgare L.) mapping populations were used to investigate the feasibility of selective genotyping for detection of quantitative trait loci (QTLs). With selective genotyping, only individuals with high and low phenotypic values for the trait of interest are genotyped. Here, genotyping of 10 to 70% of each population (i.e., 5 to 35% in each tail of the phenotypic distribution) was considered. Genomic positions detected by selective genotyping were compared to QTL position estimates from interval mapping analysis using marker genotype data from the entire population. Selective genotyping reliably detected almost all of the mapped QTLs, often with only 10% of the population genotyped. Selective genotyping also detected spurious QTLs in regions of the genome where no significant QTL had been mapped. Even with additional genotyping to verify putative QTLs, the total genotyping effort for detection of QTLs for a single trait by selective genotyping was usually less than 30% of that required for conventional interval mapping. Simultaneous investigation of two or more traits by selective genotyping would require additional genotyping effort, but could still be worthwhile.
Authors:
M Ayoub; D E Mather
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Genome / National Research Council Canada = Génome / Conseil national de recherches Canada     Volume:  45     ISSN:  0831-2796     ISO Abbreviation:  Genome     Publication Date:  2002 Dec 
Date Detail:
Created Date:  2002-12-27     Completed Date:  2003-05-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8704544     Medline TA:  Genome     Country:  Canada    
Other Details:
Languages:  eng     Pagination:  1116-24     Citation Subset:  IM    
Affiliation:
Department of Plant Science, McGill University, 21111 Lakeshore Road, Ste-Anne-de-Bellevue, QC H9X 3V9, Canada.
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MeSH Terms
Descriptor/Qualifier:
Genotype*
Hordeum / classification,  genetics*
Quantitative Trait Loci*
Species Specificity
Comments/Corrections
Erratum In:
Genome. 2006 Apr;49(4):411-2

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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