Document Detail


Effectiveness of recombinant soybean cysteine proteinase inhibitors against selected crop pests.
MedLine Citation:
PMID:  15907768     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Three recombinant soybean cysteine proteinase inhibitors (rSCPIs), L1, R1 and N2, were assessed for their potential to inhibit the growth and development of three major agricultural crop pests known to utilize digestive cysteine proteinases: Western corn rootworm (Diabrotica virgifera virgifera, WCR), Colorado potato beetle (Leptinotarsa decemlineata, CPB) and cowpea weevil (Callosobruchus maculatus, CW). In vitro experiments showed that cysteine proteinase activities in the crude gut extracts of the WCR, CPB, and CW were inhibited to various degrees by the three rSCPIs. Of the three rSCPIs tested, N2 was most effective in inhibiting the crude gut extract of WCR, CPB, and CW (50% inhibition at 5 x 10(-8), 5 x 10(-8), and 3 x 10(-7) M, respectively). The L1 was the least potent of the three CPIs tested, with 50% inhibition at 5 x 10(-6) M of the crude gut extracts of WCR. Results of in vivo experiments conducted to assess the effect of the three rSCPIs on the vital growth parameters of WCR, CPB and CW were consistent with results of the in vitro experiments.
Authors:
S Lalitha; R E Shade; L L Murdock; R A Bressan; P M Hasegawa; S S Nielsen
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Publication Detail:
Type:  Journal Article     Date:  2005-03-19
Journal Detail:
Title:  Comparative biochemistry and physiology. Toxicology & pharmacology : CBP     Volume:  140     ISSN:  1532-0456     ISO Abbreviation:  Comp. Biochem. Physiol. C Toxicol. Pharmacol.     Publication Date:  2005 Feb 
Date Detail:
Created Date:  2005-05-23     Completed Date:  2005-10-14     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100959500     Medline TA:  Comp Biochem Physiol C Toxicol Pharmacol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  227-35     Citation Subset:  IM    
Affiliation:
Department of Entomology, Purdue University, West Lafayette, IN 47907-2089, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Beetles* / growth & development
Biological Assay
Cysteine Proteinase Inhibitors* / pharmacology
Feeding Behavior / drug effects
Intestines / drug effects,  enzymology
Larva
Recombinant Proteins / pharmacology
Soybeans / chemistry*
Chemical
Reg. No./Substance:
0/Cysteine Proteinase Inhibitors; 0/Recombinant Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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