| Effectiveness of protocol for the isolation of Wharton's Jelly stem cells in large-scale applications. | |
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MedLine Citation:
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PMID: 19609625 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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The Wharton's Jelly (WJ) of the umbilical cord (UC) is an excellent source of mesenchymal stem cells (MSCs) with a range of potential therapeutic applications. The present study was conducted to demonstrate the efficiency of the protocols used by Biogenea-Cellgenea Ltd. for isolation and expansion of WJ MSCs from donors across Greece. Umbilical cord samples were collected from 599 females following childbirth and processed for WJ MSC isolation. Stem cells were expanded using DMEM-based media and cell counts and overall viability figures derived using Trypan blue exclusion. To investigate the application of isolation and expansion protocols on samples received 1, 2, 3, 4 and 5 d after their collection, ten fresh samples were processed at these time intervals and evaluated. The cellular yield of most WJ samples was 1.1–5.0×10(6) cells at 21–30 d after processing. As culture time increased, cell counts decreased. Statistical analysis of mean cell counts showed a significant reduction after 21 d. Finally, we demonstrate for the first time that it is possible to obtain satisfactory cell numbers from samples processed 1, 2, 3, 4 and even 5 d after collection. We have derived favourable data on the protocols used at Biogenea-Cellgenea Ltd. to isolate and culture MSCs from the WJ. Protocol choice is crucial when handling large numbers of samples on a daily basis and should be made to ensure the best possible outcome. |
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Authors:
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Anastasia Petsa; Sofia Gargani; Antigonos Felesakis; Nikolaos Grigoriadis; Ioannis Grigoriadis |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: In vitro cellular & developmental biology. Animal Volume: 45 ISSN: 1543-706X ISO Abbreviation: In Vitro Cell. Dev. Biol. Anim. Publication Date: 2009 Dec |
Date Detail:
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Created Date: 2011-04-07 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9418515 Medline TA: In Vitro Cell Dev Biol Anim Country: Germany |
Other Details:
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Languages: eng Pagination: 573-6 Citation Subset: IM |
Affiliation:
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Biogenea-Cellgenea Ltd., Plateia Business & Trade Centre, Building A, 5th Floor, Tsimiski 43, Thessaloniki 546 23, Greece. anastasia.petsa@gmail.com |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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