| Effectiveness of ischemic preconditioning on long-term myocardial preservation. | |
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MedLine Citation:
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PMID: 9583859 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: This study was designed to assess whether the protective effect of ischemic preconditioning can be adapted for myocardium undergoing 6 hr of ischemia. METHODS: Eighteen isolated rat hearts were perfused with oxygen-bicarbonated Krebs-Henseleit buffer in the Langendorff mode for 35 min (group A, controls) or perfused in the Langendorff apparatus for 20 min, followed by 5 min of global normothermic ischemia and 10 min of buffer perfusion (group B, preconditioning) or followed by two cycles of 2.5 min of global normothermic ischemia plus 5 min of buffer perfusion (group C, preconditioning). The hearts were then arrested and preserved for 6 hr with Bretschneider's histidine-tryptophan-potassium cardioplegic solution at 4 degrees C, followed by 30 min of reperfusion. Recovery of cardiac function, postischemic enzyme leakage, and intracellular calcium concentration were compared. RESULTS: After 6 hr of ischemia, the hearts that underwent preconditioning in groups B and C showed better recovery of left ventricular developed pressure (P<0.05), a lower end-diastolic pressure level (P<0.05), less leakage of creatine kinase, and a lower intracellular calcium concentration than those in group A. There were no statistical differences in the rate of recovery of coronary flow. CONCLUSIONS: Our study demonstrated that ischemic preconditioning improves myocardial functional recovery after 6 hr of hypothermic preservation in the isolated rat heart. Preconditioning might be useful for preserving the heart against long-term ischemia/reperfusion injury. |
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Authors:
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M Hachida; H Lu; A Ohkado; H Hoshi; H Gu; T Nakanishi; H Koyanagi |
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Publication Detail:
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Type: In Vitro; Journal Article |
Journal Detail:
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Title: Transplantation Volume: 65 ISSN: 0041-1337 ISO Abbreviation: Transplantation Publication Date: 1998 Apr |
Date Detail:
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Created Date: 1998-05-22 Completed Date: 1998-05-22 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0132144 Medline TA: Transplantation Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1021-4 Citation Subset: IM |
Affiliation:
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Department of Cardiovascular Surgery, The Heart Institute of Japan, Tokyo Women's Medical College, Shinjuku. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Calcium / metabolism Cardioplegic Solutions Coronary Circulation / physiology* Creatine Kinase / analysis Glucose Heart* / physiology Ischemic Preconditioning, Myocardial* Mannitol Myocardial Ischemia Myocardial Reperfusion Organ Preservation* Potassium Chloride Procaine Rats Ventricular Function, Left |
| Chemical | |
Reg. No./Substance:
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0/Bretschneider cardioplegic solution; 0/Cardioplegic Solutions; 50-99-7/Glucose; 59-46-1/Procaine; 69-65-8/Mannitol; 7440-70-2/Calcium; 7447-40-7/Potassium Chloride; EC 2.7.3.2/Creatine Kinase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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