Document Detail

Effectiveness of ischemic preconditioning on long-term myocardial preservation.
MedLine Citation:
PMID:  9583859     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: This study was designed to assess whether the protective effect of ischemic preconditioning can be adapted for myocardium undergoing 6 hr of ischemia. METHODS: Eighteen isolated rat hearts were perfused with oxygen-bicarbonated Krebs-Henseleit buffer in the Langendorff mode for 35 min (group A, controls) or perfused in the Langendorff apparatus for 20 min, followed by 5 min of global normothermic ischemia and 10 min of buffer perfusion (group B, preconditioning) or followed by two cycles of 2.5 min of global normothermic ischemia plus 5 min of buffer perfusion (group C, preconditioning). The hearts were then arrested and preserved for 6 hr with Bretschneider's histidine-tryptophan-potassium cardioplegic solution at 4 degrees C, followed by 30 min of reperfusion. Recovery of cardiac function, postischemic enzyme leakage, and intracellular calcium concentration were compared. RESULTS: After 6 hr of ischemia, the hearts that underwent preconditioning in groups B and C showed better recovery of left ventricular developed pressure (P<0.05), a lower end-diastolic pressure level (P<0.05), less leakage of creatine kinase, and a lower intracellular calcium concentration than those in group A. There were no statistical differences in the rate of recovery of coronary flow. CONCLUSIONS: Our study demonstrated that ischemic preconditioning improves myocardial functional recovery after 6 hr of hypothermic preservation in the isolated rat heart. Preconditioning might be useful for preserving the heart against long-term ischemia/reperfusion injury.
M Hachida; H Lu; A Ohkado; H Hoshi; H Gu; T Nakanishi; H Koyanagi
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Transplantation     Volume:  65     ISSN:  0041-1337     ISO Abbreviation:  Transplantation     Publication Date:  1998 Apr 
Date Detail:
Created Date:  1998-05-22     Completed Date:  1998-05-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0132144     Medline TA:  Transplantation     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1021-4     Citation Subset:  IM    
Department of Cardiovascular Surgery, The Heart Institute of Japan, Tokyo Women's Medical College, Shinjuku.
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MeSH Terms
Calcium / metabolism
Cardioplegic Solutions
Coronary Circulation / physiology*
Creatine Kinase / analysis
Heart* / physiology
Ischemic Preconditioning, Myocardial*
Myocardial Ischemia
Myocardial Reperfusion
Organ Preservation*
Potassium Chloride
Ventricular Function, Left
Reg. No./Substance:
0/Bretschneider cardioplegic solution; 0/Cardioplegic Solutions; 50-99-7/Glucose; 59-46-1/Procaine; 69-65-8/Mannitol; 7440-70-2/Calcium; 7447-40-7/Potassium Chloride; EC Kinase

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