| Effectiveness of Brucella abortus lipopolysaccharide as an adjuvant for tuberculin PPD. | |
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MedLine Citation:
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PMID: 20965746 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Bacterial lipopolysaccharide (LPS) has T-helper 1 (Th1) immunostimulatory activities but because of toxicity and pyrogenicity cannot be used as an adjuvant. Brucella abortus LPS has less toxicity and no pyrogenic properties in comparison to other bacterial LPS. In the current study, the immunostimulatory properties of B. abortus LPS were evaluated for its adjuvant activity. Tuberculin purified protein derivative (PPD) from Mycobacterium tuberculosis was extracted and after anion-exchange chromatography on Q-sepharose column, two fractions (17 and 23), which dominantly contained 30- and 70-kDa antigens, were collected for immunological studies. BALB/c mice were immunized with four different antigen preparations (BCG, PPD, 17th and 23rd PPD fractions) along with complete Freund's adjuvant or B. abortus LPS. The T-cell immune response of mice was assessed by measurement of Th1-type cytokine (IFN-γ) and Th2-type cytokines (IL-5 and IL-10) levels. Also, the humoral immunity was evaluated by measuring the specific IgG levels. Our results showed that immunization of mice with 17th PPD fraction along with B. abortus LPS can induce a Th1-type cytokine response characterized with a high IFN-γ/IL-5 ratio, while immunization with PPD or 23rd PPD fraction along with the same adjuvant resulted to a mixed Th1/Th2-type cytokine response. |
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Authors:
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Mostafa Jamalan; Susan Kaboudanian Ardestani; Majid Zeinali; Nader Mosaveri; Mohammad Mohammad Taheri |
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Publication Detail:
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Type: Journal Article Date: 2010-10-20 |
Journal Detail:
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Title: Biologicals : journal of the International Association of Biological Standardization Volume: 39 ISSN: 1095-8320 ISO Abbreviation: Biologicals Publication Date: 2011 Jan |
Date Detail:
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Created Date: 2011-02-07 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9004494 Medline TA: Biologicals Country: England |
Other Details:
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Languages: eng Pagination: 23-8 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 The International Association for Biologicals. Published by Elsevier Ltd. All rights reserved. |
Affiliation:
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Immunology Lab, Institute of Biochemistry and Biophysics, University of Tehran, Enghelab Ave, P.O. Box 13145-1384, Tehran, Iran. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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