Document Detail

Effective stage in the cell cycle for control of the budding direction of cdc mutants of Saccharomyces cerevisiae using electric stimulus.
MedLine Citation:
PMID:  3048417     Owner:  NLM     Status:  MEDLINE    
Cell division cycle (cdc) mutants of Saccharomyces cerevisiae were used to determine the most effective stage for the directional control of cell budding using an electric stimulus. The selected mutants were cdc 35 and cdc 28, which could be reversibly arrested before spindle pole body satellite formation (SPBSF) and spindle pole body duplication (SPBD), respectively. The budding direction (theta) was defined so that the direction parallel to that of the electric field was 0 degree. Considering the symmetry of the experimental conditions, the range of theta was defined as 0-90 degrees. The electric stimulus applied in the present study was alternating pulses (pulse height, +/- 15 V; pulse width at half pulse height, 5 microseconds; frequency; 10 kHz). The peak height of the cross membrane potential was estimated as 472 mV, which was sufficient to induce considerable strain in the cell membrane. In the case of cdc 35, the 95% confidence interval (95% CI) of the budding direction was 7-25 degrees when subjected to electric stimulus, while the 95% CI of the budding direction without electric stimulus was 35-57 degrees. In the case of cdc 28, 95% CI values of the budding direction with and without electric stimulus were 1229 degrees and 23-56 degrees, respectively. These results demonstrate that the stage after SPBD is effective for the directional control of yeast cell budding using an electric stimulus. Simultaneously, an electric stimulus reduced the cell budding time of both the cdc mutants used. Therefore, the electric stimulus was also effective in promoting cell cycle progression under the present conditions.
H Matsuoka; S Matsumoto; M Kinoshita; S Yamada
Related Documents :
18590977 - Efficiency of cytoplasmic delivery by non-cationic liposomes to cells in vitro: a confo...
10949997 - Differentiation of myoblasts is accelerated in culture in a magnetic field.
2420807 - Motility of cultured fish epidermal cells in the presence and absence of direct current...
17268627 - Continuous dielectrophoretic cell separation microfluidic device.
17601877 - Regulation of phosphoinositide levels by the phospholipid transfer protein sec14p contr...
3709967 - Acquisition of developmental autonomy in the equatorial region of the xenopus embryo.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  971     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1988 Oct 
Date Detail:
Created Date:  1988-11-16     Completed Date:  1988-11-16     Revised Date:  2000-12-18    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  255-65     Citation Subset:  IM    
Department of Industrial Chemistry, Faculty of Technology, Tokyo University of Agriculture and Technology, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Cell Cycle
Electric Stimulation
Electrophysiology / instrumentation,  methods
Membrane Potentials
Models, Theoretical
Saccharomyces cerevisiae / cytology,  genetics,  growth & development*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Identity of soluble thiamin-binding protein with thiamin-repressible acid phosphatase in Saccharomyc...
Next Document:  Characterization of vesicles containing insulin-responsive intracellular glucose transporters isolat...