Document Detail

Effective purging of bone marrow by a combination of immunorosette depletion and complement lysis.
MedLine Citation:
PMID:  2298269     Owner:  NLM     Status:  MEDLINE    
The effectiveness of a simple immunorosette technique for the depletion of common acute lymphatic leukemic (cALL) blasts from autologous bone marrow transplants was studied. Erythrocytes were sensitized with tetramolecular complexes consisting of rat anti-mouse IgG1 monoclonal antibodies (McAbs) that crosslink two different mouse McAbs. One of the McAbs was directed against glycophorin A, and the other was directed against marker glycoproteins of B cells and their precursors (CD9, CD10, CD19, or CD22). Immunorosettes were formed by addition of the sensitized erythrocytes to the cALL+ cells. After density-gradient separation of immunorosettes from mixtures of cALL+/terminal deoxynucleotidyl transferase-positive (TdT+) leukemic blasts and mononuclear bone marrow cells, nearly a 2-log depletion of leukemic cells was measured by flow cytometry. Clonogenic assays with two cALL+B-cell lines (Ros-17 and Nalm-16) were performed to compare the efficacy of complement-mediated cell lysis, immunorosette depletion, and a combination of both procedures. Complement-mediated cytotoxicity with the three McAbs in combination with baby rabbit complement yielded a 1- to 2-log cell kill. Immunorosette depletion resulted in a 3-log reduction of clonogenic units. Sequential application of the two methods (immunorosette depletion with CD19 McAb followed by a complement lysis with CD9 and CD10 McAbs) led to superior results in causing a 4- to 5-log purging effect. These purging procedures did not cause a loss of normal myeloid (granulocyte-macrophage colony-forming units, CFU-GM) or erythroid (erythroid burst-forming units, BFU-e) progenitors from the bone marrow. This study indicates that the combination of the two methods results in a highly efficient purging procedure for the removal of cALL+ cells from autologous bone marrow cells.
I C Slaper-Cortenbach; L G Admiraal; E F van Leeuwen; J M Kerr; A E von dem Borne; P A Tetteroo
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Experimental hematology     Volume:  18     ISSN:  0301-472X     ISO Abbreviation:  Exp. Hematol.     Publication Date:  1990 Jan 
Date Detail:
Created Date:  1990-02-23     Completed Date:  1990-02-23     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0402313     Medline TA:  Exp Hematol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  49-54     Citation Subset:  IM    
Department of Immunological Hematology, Central Laboratory of the Netherlands Red Cross Blood Transfusion Service, Amsterdam, The Netherlands.
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MeSH Terms
Antibodies, Monoclonal / immunology
Bone Marrow / pathology*
Bone Marrow Transplantation
Complement System Proteins / immunology*
Cytotoxicity, Immunologic*
Flow Cytometry
Hematopoietic Stem Cells
Neoplastic Stem Cells / pathology
Precursor Cell Lymphoblastic Leukemia-Lymphoma / pathology*
Rosette Formation*
Tumor Cells, Cultured
Reg. No./Substance:
0/Antibodies, Monoclonal; 9007-36-7/Complement System Proteins

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