| Effective inhibition of porcine transmissible gastroenteritis virus replication in ST cells by shRNAs targeting RNA-dependent RNA polymerase gene. | |
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MedLine Citation:
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PMID: 17287033 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Transmissible gastroenteritis virus (TGEV) is identified as one of the most important pathogenic agents during swine enteric infection, leading to high mortality in neonatal pigs and severe annual economic loss in swine-producing areas. Up to date, various vaccines developed against TGEV still need to be improved. To exploit the possibility of using RNA interference (RNAi) as a strategy against TGEV infection, two shRNA-expressing plasmids (pEGFP-U6/P1 and pEGFP-U6/P2) targeting the RNA-dependent RNA polymerase (RdRp) gene of TGEV were constructed and transfected into swine testicular (ST) cells. The cytopathic effect (CPE) and MTS assays demonstrated that both shRNAs were capable of protecting cells against TGEV invasion with very high specificity and efficiency. A real-time quantitative RT-PCR further confirmed that the amounts of viral RNAs in cell cultures pre-transfected with the two plasmids were reduced by 95.2% and up to 100%, respectively. Our results suggest that RNAi might be a promising new strategy against TGEV infection. |
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Authors:
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Jun-fang Zhou; Xiu-guo Hua; Li Cui; Jian-guo Zhu; De-nian Miao; Yong Zou; Xi-zhong He; Wan-guo Su |
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Publication Detail:
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Type: Journal Article Date: 2007-01-25 |
Journal Detail:
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Title: Antiviral research Volume: 74 ISSN: 0166-3542 ISO Abbreviation: Antiviral Res. Publication Date: 2007 Apr |
Date Detail:
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Created Date: 2007-03-12 Completed Date: 2007-07-23 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8109699 Medline TA: Antiviral Res Country: Netherlands |
Other Details:
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Languages: eng Pagination: 36-42 Citation Subset: IM |
Affiliation:
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Department of Animal Science, School of Agriculture and Biology, Shanghai Jiao Tong University, 2678 Qixin Road, Shanghai 201101, China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Line / virology Gastroenteritis, Transmissible, of Swine / therapy, virology* Genes, Viral* Plasmids RNA Interference* RNA Replicase / genetics* Sensitivity and Specificity Swine Transfection / methods Transmissible gastroenteritis virus / physiology* Virus Replication / genetics |
| Chemical | |
Reg. No./Substance:
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EC 2.7.7.48/RNA Replicase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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