Document Detail


Effective inhibition of porcine transmissible gastroenteritis virus replication in ST cells by shRNAs targeting RNA-dependent RNA polymerase gene.
MedLine Citation:
PMID:  17287033     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Transmissible gastroenteritis virus (TGEV) is identified as one of the most important pathogenic agents during swine enteric infection, leading to high mortality in neonatal pigs and severe annual economic loss in swine-producing areas. Up to date, various vaccines developed against TGEV still need to be improved. To exploit the possibility of using RNA interference (RNAi) as a strategy against TGEV infection, two shRNA-expressing plasmids (pEGFP-U6/P1 and pEGFP-U6/P2) targeting the RNA-dependent RNA polymerase (RdRp) gene of TGEV were constructed and transfected into swine testicular (ST) cells. The cytopathic effect (CPE) and MTS assays demonstrated that both shRNAs were capable of protecting cells against TGEV invasion with very high specificity and efficiency. A real-time quantitative RT-PCR further confirmed that the amounts of viral RNAs in cell cultures pre-transfected with the two plasmids were reduced by 95.2% and up to 100%, respectively. Our results suggest that RNAi might be a promising new strategy against TGEV infection.
Authors:
Jun-fang Zhou; Xiu-guo Hua; Li Cui; Jian-guo Zhu; De-nian Miao; Yong Zou; Xi-zhong He; Wan-guo Su
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Publication Detail:
Type:  Journal Article     Date:  2007-01-25
Journal Detail:
Title:  Antiviral research     Volume:  74     ISSN:  0166-3542     ISO Abbreviation:  Antiviral Res.     Publication Date:  2007 Apr 
Date Detail:
Created Date:  2007-03-12     Completed Date:  2007-07-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8109699     Medline TA:  Antiviral Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  36-42     Citation Subset:  IM    
Affiliation:
Department of Animal Science, School of Agriculture and Biology, Shanghai Jiao Tong University, 2678 Qixin Road, Shanghai 201101, China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line / virology
Gastroenteritis, Transmissible, of Swine / therapy,  virology*
Genes, Viral*
Plasmids
RNA Interference*
RNA Replicase / genetics*
Sensitivity and Specificity
Swine
Transfection / methods
Transmissible gastroenteritis virus / physiology*
Virus Replication / genetics
Chemical
Reg. No./Substance:
EC 2.7.7.48/RNA Replicase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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