| Effective inhibition of melanosome transfer to keratinocytes by lectins and niacinamide is reversible. | |
| | |
MedLine Citation:
|
PMID: 15946237 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Skin pigmentation results in part from the transfer of melanized melanosomes synthesized by melanocytes to neighboring keratinocytes. Plasma membrane lectins and their glycoconjugates expressed by these epidermal cells are critical molecules involved in this transfer process. In addition, the derivative of vitamin B(3), niacinamide, can inhibit melanosome transfer and induce skin lightening. We investigated the effects of these molecules on the viability of melanocytes and keratinocytes and on the reversibility of melanosome-transfer inhibition induced by these agents using an in vitro melanocyte-keratinocyte coculture model system. While lectins and neoglycoproteins could induce apoptosis in a dose-dependent manner to melanocytes or keratinocytes in monoculture, similar dosages of the lectins, as opposed to neoglycoproteins, did not induce apoptosis to either cell type when treated in coculture. The dosages of lectins and niacinamide not affecting cell viability produced an inhibitory effect on melanosome transfer, when used either alone or together in cocultures of melanocytes-keratinocytes. Cocultures treated with lectins or niacinamide resumed normal melanosome transfer in 3 days after removal of the inhibitor, while cocultures treated with a combination of lectins and niacinamide demonstrated a lag in this recovery. Subsequently, we assessed the effect of niacinamide on facial hyperpigmented spots using a vehicle-controlled, split-faced design human clinical trial. Topical application of niacinamide resulted in a dose-dependent and reversible reduction in hyperpigmented lesions. These results suggest that lectins and niacinamide at concentrations that do not affect cell viability are reversible inhibitors of melanosome transfer. |
| | |
Authors:
|
Amanda Greatens; Tomohiro Hakozaki; Amy Koshoffer; Howard Epstein; Sandy Schwemberger; George Babcock; Donald Bissett; Hirotsugu Takiwaki; Seiji Arase; R Randall Wickett; Raymond E Boissy |
Related Documents
:
|
908977 - Iminopeptiduria, skin ulcerations, and edema in a boy with prolidase deficiency. 12861857 - Does skin prick test correlate with basophil-associated mite-specific ige in atopic chi... 2649607 - The role of langerhans cells in allergic contact hypersensitivity. a review of findings... 1981417 - Reactive changes in the langerhans' cells of human skin caused by occlusion with water ... 3427217 - Metabolic integrity and stability of isolated rat brown adipocytes. 7330857 - Epidermal feet in pupal segment morphogenesis. |
Publication Detail:
|
Type: Clinical Trial; Journal Article; Randomized Controlled Trial; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Experimental dermatology Volume: 14 ISSN: 0906-6705 ISO Abbreviation: Exp. Dermatol. Publication Date: 2005 Jul |
Date Detail:
|
Created Date: 2005-06-10 Completed Date: 2005-09-14 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 9301549 Medline TA: Exp Dermatol Country: Denmark |
Other Details:
|
Languages: eng Pagination: 498-508 Citation Subset: IM |
Affiliation:
|
Department of Dermatology, University of Cincinnati, Cincinnati, OH, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Administration, Topical Adult Cell Survival / drug effects Cells, Cultured Coculture Techniques Double-Blind Method Female Humans Hyperpigmentation / drug therapy, metabolism, pathology Keratinocytes / cytology, drug effects*, metabolism* Lectins / pharmacology* Melanocytes / cytology, drug effects, metabolism Melanosomes / drug effects*, metabolism* Middle Aged Niacinamide / administration & dosage, pharmacology*, therapeutic use Skin Pigmentation / drug effects, physiology |
| Chemical | |
Reg. No./Substance:
|
0/Lectins; 98-92-0/Niacinamide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Expression of melanoma-associated antigens in melanoma cell cultures.
Next Document: Induction of connective tissue growth factor expression by sphingosylphosphorylcholine in cultured h...