Document Detail


Effective inhibition of melanosome transfer to keratinocytes by lectins and niacinamide is reversible.
MedLine Citation:
PMID:  15946237     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Skin pigmentation results in part from the transfer of melanized melanosomes synthesized by melanocytes to neighboring keratinocytes. Plasma membrane lectins and their glycoconjugates expressed by these epidermal cells are critical molecules involved in this transfer process. In addition, the derivative of vitamin B(3), niacinamide, can inhibit melanosome transfer and induce skin lightening. We investigated the effects of these molecules on the viability of melanocytes and keratinocytes and on the reversibility of melanosome-transfer inhibition induced by these agents using an in vitro melanocyte-keratinocyte coculture model system. While lectins and neoglycoproteins could induce apoptosis in a dose-dependent manner to melanocytes or keratinocytes in monoculture, similar dosages of the lectins, as opposed to neoglycoproteins, did not induce apoptosis to either cell type when treated in coculture. The dosages of lectins and niacinamide not affecting cell viability produced an inhibitory effect on melanosome transfer, when used either alone or together in cocultures of melanocytes-keratinocytes. Cocultures treated with lectins or niacinamide resumed normal melanosome transfer in 3 days after removal of the inhibitor, while cocultures treated with a combination of lectins and niacinamide demonstrated a lag in this recovery. Subsequently, we assessed the effect of niacinamide on facial hyperpigmented spots using a vehicle-controlled, split-faced design human clinical trial. Topical application of niacinamide resulted in a dose-dependent and reversible reduction in hyperpigmented lesions. These results suggest that lectins and niacinamide at concentrations that do not affect cell viability are reversible inhibitors of melanosome transfer.
Authors:
Amanda Greatens; Tomohiro Hakozaki; Amy Koshoffer; Howard Epstein; Sandy Schwemberger; George Babcock; Donald Bissett; Hirotsugu Takiwaki; Seiji Arase; R Randall Wickett; Raymond E Boissy
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Publication Detail:
Type:  Clinical Trial; Journal Article; Randomized Controlled Trial; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Experimental dermatology     Volume:  14     ISSN:  0906-6705     ISO Abbreviation:  Exp. Dermatol.     Publication Date:  2005 Jul 
Date Detail:
Created Date:  2005-06-10     Completed Date:  2005-09-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9301549     Medline TA:  Exp Dermatol     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  498-508     Citation Subset:  IM    
Affiliation:
Department of Dermatology, University of Cincinnati, Cincinnati, OH, USA.
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MeSH Terms
Descriptor/Qualifier:
Administration, Topical
Adult
Cell Survival / drug effects
Cells, Cultured
Coculture Techniques
Double-Blind Method
Female
Humans
Hyperpigmentation / drug therapy,  metabolism,  pathology
Keratinocytes / cytology,  drug effects*,  metabolism*
Lectins / pharmacology*
Melanocytes / cytology,  drug effects,  metabolism
Melanosomes / drug effects*,  metabolism*
Middle Aged
Niacinamide / administration & dosage,  pharmacology*,  therapeutic use
Skin Pigmentation / drug effects,  physiology
Chemical
Reg. No./Substance:
0/Lectins; 98-92-0/Niacinamide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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