Document Detail


Effective chemical preservation of morphology of urinary erythrocytes.
MedLine Citation:
PMID:  12748844     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Between 2 and 4 h after miction the morphology of urinary erythrocytes has changed, clouding the distinction between glomerular and non-glomerular bleeding in pediatric patients. Glomerular bleeding is characterized by microscopically visible alteration in urinary erythrocytes due to glomerular disease. Fixation by Cellfix, a formaldehyde-based fixative, allows the preservation of the morphology for at least 24 h and can be recommended for clinical practice. In our experience, thiomersal was not effective for preservation of the morphology of erythrocytes.
Authors:
Olga Komarova; Wim van der Meer; Elena Levtchenko; Leo Monnens
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Publication Detail:
Type:  Journal Article     Date:  2003-05-14
Journal Detail:
Title:  Pediatric nephrology (Berlin, Germany)     Volume:  18     ISSN:  0931-041X     ISO Abbreviation:  Pediatr. Nephrol.     Publication Date:  2003 Jul 
Date Detail:
Created Date:  2003-06-12     Completed Date:  2004-03-01     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8708728     Medline TA:  Pediatr Nephrol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  665-6     Citation Subset:  IM    
Affiliation:
Department of Pediatric Nephrology, Institute of Pediatrics, RAMN, Moscow, Russia.
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MeSH Terms
Descriptor/Qualifier:
Acanthocytes / ultrastructure
Child
Erythrocytes / ultrastructure*
Fixatives
Formaldehyde
Glomerulonephritis / blood,  urine
Glomerulonephritis, IGA / blood,  urine
Glomerulonephritis, Membranoproliferative / blood,  urine
Hematuria / blood*
Humans
Microscopy, Phase-Contrast
Specimen Handling / methods*
Time Factors
Tissue Fixation / methods*
Chemical
Reg. No./Substance:
0/Fixatives; 50-00-0/Formaldehyde

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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