Document Detail


Effective cryopreservation of human embryonic stem cells by programmed freezing.
MedLine Citation:
PMID:  17282221     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
Cryopreservation of human embryonic stem cells is an important and unsolved problem. A computer-controlled programmable cooler is used in the preservation of ES cells. Several effects have been experimentally studied, which include the cooling rate, the seeding temperature, the terminative temperature before the sample is plunged into liquid nitrogen. It is found that the constitution of cryoprotective agents is Me2SO+FBS+DMEM(1:3:6,v/v/v), and the sample is cooled from 0 °C to -35 °C with a cooling rate of 0.5 °C/min (seeding at -10 °C), and then being plunged into the liquid nitrogen immediately. The high survival rate (81.8%) is obtained. The cryopreserved human ES cells have been cultivated for prolonged periods and retained the properties of pluripotent cells, they have a normal karyotype and show the histochemical staining for alkaline phosphatase.
Authors:
Peng-Fei Yang; Tse-Chao Hua; H-C Tsung; Qi-Kang Cheng; Yi-Lin Cao
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference     Volume:  1     ISSN:  1557-170X     ISO Abbreviation:  Conf Proc IEEE Eng Med Biol Soc     Publication Date:  2005  
Date Detail:
Created Date:  2007-02-06     Completed Date:  2012-10-02     Revised Date:  2014-08-21    
Medline Journal Info:
Nlm Unique ID:  101243413     Medline TA:  Conf Proc IEEE Eng Med Biol Soc     Country:  United States    
Other Details:
Languages:  eng     Pagination:  482-5     Citation Subset:  -    
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