| Effect of xanthohumol and isoxanthohumol on 3T3-L1 cell apoptosis and adipogenesis. | |
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MedLine Citation:
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PMID: 17874298 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Xanthohumol (XN), the chalcone from beer hops has several biological activities. XN has been shown to induce apoptosis in cancer cells and also has been reported to be involved in lipid metabolism. Based on these studies and our previous work with natural compounds, we hypothesized that XN and its isomeric flavanone, isoxanthohumol (IXN), would induce apoptosis in adipocytes through the mitochondrial pathway and would inhibit maturation of preadipocytes. Adipocytes were treated with various concentrations of XN or IXN. In mature adipocytes both XN and IXN decreased viability, increased apoptosis and increased ROS production, XN being more effective. Furthermore, the antioxidants ascorbic acid and 2-mercaptoethanol prevented XN and IXN-induced ROS generation and apoptosis. Immunoblotting analysis showed an increase in the levels of cytoplasmic cytochrome c and cleaved poly (ADP-ribose) polymerase (PARP) by XN and IXN. Concomitantly, we observed activation of the effectors caspase-3/7. In maturing preadipocytes both XN and IXN were effective in reducing lipid content, XN being more potent. Moreover, the major adipocyte marker proteins such as PPARgamma, C/EBPalpha, and aP2 decreased after treatment with XN during the maturation period and that of DGAT1 decreased after treatment with XN and IXN. Taken together, our data indicate that both XN and IXN inhibit differentiation of preadipocytes, and induce apoptosis in mature adipocytes, but XN is more potent. |
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Authors:
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Jeong-Yeh Yang; Mary Anne Della-Fera; Srujana Rayalam; Clifton A Baile |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Apoptosis : an international journal on programmed cell death Volume: 12 ISSN: 1360-8185 ISO Abbreviation: Apoptosis Publication Date: 2007 Nov |
Date Detail:
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Created Date: 2007-10-01 Completed Date: 2007-12-21 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9712129 Medline TA: Apoptosis Country: United States |
Other Details:
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Languages: eng Pagination: 1953-63 Citation Subset: IM |
Affiliation:
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Department of Animal and Dairy Science, 444 Edgar L. Rhodes Center for Animal and Dairy Science, University of Georgia, Athens, GA 30602-2771, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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3T3-L1 Cells Adipogenesis / drug effects* Animals Apoptosis / drug effects* Cell Differentiation / drug effects Cell Survival / drug effects Fibroblasts / cytology, drug effects* Growth Inhibitors / pharmacology Humulus Mice Propiophenones / pharmacology* Xanthones / pharmacology* |
| Chemical | |
Reg. No./Substance:
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0/Growth Inhibitors; 0/Propiophenones; 0/Xanthones; 0/isoxanthohumol; 6754-58-1/xanthohumol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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