| Effect of water-soluble P-chitosan and S-chitosan on human primary osteoblasts and giant cell tumor of bone stromal cells. | |
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MedLine Citation:
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PMID: 21205994 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Water-soluble phosphorylated chitosan (P-chitosan) and disodium (1 → 4)-2-deoxy-2-sulfoamino-β-D-glucopyranuronan (S-chitosan) are two chemically modified chitosans. In this study, we found that P-chitosan significantly promotes cell proliferation of both human primary osteoblasts (OBs) and the OB like stromal cell component of the giant cell tumor of bone (GCTB) cells at the concentration from 125 to 1000 µg ml(-1) at all time points of 1, 3, 5 and 7 days after treatment. Further investigation of the osteogenic effect of the P-chitosan suggested that it regulates the levels of osteoclastogenic factors, receptor activator of nuclear factor kappa B ligand and osteoprotegerin expression. An interesting finding is that S-chitosan at lower concentration (100 µg ml(-1)) stimulates cell proliferation while a higher dose (1000 µg ml(-1)) of S-chitosan inhibits it. The inhibitory effect of S-chitosan on human primary GCT stromal cells was greater than that of OBs (p < 0.05). Taken together, our findings elucidated the osteogenic effect of P-chitosan and the varying effects of S-chitosan on the proliferation of human primary OBs and GCT stromal cells and provided us the rationale for the construction of novel bone repair biomaterials with the dual properties of bone induction and bone tumor inhibition. |
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Authors:
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T Tang; G Zhang; Carol Py Lau; L Z Zheng; X H Xie; X L Wang; X H Wang; K He; Y Patrick; L Qin; Shekhar M Kumta |
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Publication Detail:
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Type: Journal Article Date: 2011-01-05 |
Journal Detail:
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Title: Biomedical materials (Bristol, England) Volume: 6 ISSN: 1748-605X ISO Abbreviation: Biomed Mater Publication Date: 2011 Feb |
Date Detail:
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Created Date: 2011-01-26 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101285195 Medline TA: Biomed Mater Country: England |
Other Details:
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Languages: eng Pagination: 015004 Citation Subset: IM |
Affiliation:
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Department of Orthopaedics & Traumatology, The Chinese University of Hong Kong, Hong Kong, People's Republic of China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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