Document Detail

Effect of vitrectomy and cytopreparatory techniques on cell survival and preservation.
MedLine Citation:
PMID:  1633587     Owner:  NLM     Status:  MEDLINE    
Obtaining vitreous fluid by means of vitrectomy frequently results in a specimen that is difficult to assess cytologically. We devised an experimental model to examine the effect of the vitrector on human leukemic cancer (HL60) cells in suspension and to evaluate the cytopreparatory techniques of membrane filtration and cytocentrifugation. Eighteen 3-mL specimens of cells at concentrations ranging from 1 to 9 x 10(5)/mL were vitrectomized, and eighteen 3-mL control samples matched for cell concentration were obtained atraumatically. No significant difference in cell loss, as determined by means of staining with nigrosin vital dye, was found at any cell concentration between the vitrectomized and control specimens. The specimens were then processed cytologically. On cytologic assessment it was not possible to distinguish the vitrectomized and control specimens. A higher degree of cell preservation was noted at higher cell concentrations regardless of the cytopreparatory technique, but at lower concentrations membrane filtration resulted in a higher proportion of cytologically assessable specimens than did cytocentrifugation (42% vs. 22%). The results suggest that the vitrector causes minimal cellular damage and that to obtain optimal results both cytopreparatory techniques should be used with all vitrectomy specimens.
M R Conlon; I Craig; J F Harris; P Molinaro; M Ventresca; J R Gonder
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Canadian journal of ophthalmology. Journal canadien d'ophtalmologie     Volume:  27     ISSN:  0008-4182     ISO Abbreviation:  Can. J. Ophthalmol.     Publication Date:  1992 Jun 
Date Detail:
Created Date:  1992-08-25     Completed Date:  1992-08-25     Revised Date:  2000-12-18    
Medline Journal Info:
Nlm Unique ID:  0045312     Medline TA:  Can J Ophthalmol     Country:  CANADA    
Other Details:
Languages:  eng     Pagination:  168-71     Citation Subset:  IM    
Department of Ophthalmology, University of Western Ontario, London.
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MeSH Terms
Cell Count
Cell Survival
Cytological Techniques
Organ Preservation
Tumor Cells, Cultured
Vitreous Body / cytology*

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