| Effect of vitamins C and E on endogenous synthesis of N-nitrosamino acids in humans: precursor-product studies with [15N]nitrate. | |
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MedLine Citation:
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PMID: 4063996 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The endogenous formation of nitrosoproline (NPRO) following administration of nitrate and proline is reported in ten healthy young adults. There was a relatively constant basal excretion of NPRO, 26 +/- 10 (SD) nmol/day, in excess of amounts found in the diet. This basal synthesis of NPRO was not reduced by ascorbic acid (2 g/day) or alpha-tocopherol (400 mg/day). A significant rise in the excretion of NPRO was observed following the administration of nitrate and proline, ranging from 29 to 318 nmol/24 h with a mean of 100 nmol/24 h. [15N]Nitrate was used as a tracer to study the observed excess excretion of NPRO in urine. The data revealed that urinary NPRO excretion as a result of endogenous synthesis is not totally derived from ingested nitrate as its precursor. The ingestion of ascorbic acid and alpha-tocopherol inhibited the incorporation of [15N]nitrate into NPRO by 81 and 59%, respectively. An additional nitrosamino acid, N-nitrosothiazolidine-4-carboxylic acid, was present in the urine. It was found that N-nitrosothiazolidine-4-carboxylic acid increased 6-fold upon ingestion of nitrate. Ascorbic acid and alpha-tocopherol blocked this nitrate induced synthesis. |
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Authors:
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D A Wagner; D E Shuker; C Bilmazes; M Obiedzinski; I Baker; V R Young; S R Tannenbaum |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Cancer research Volume: 45 ISSN: 0008-5472 ISO Abbreviation: Cancer Res. Publication Date: 1985 Dec |
Date Detail:
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Created Date: 1985-12-30 Completed Date: 1985-12-30 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 2984705R Medline TA: Cancer Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 6519-22 Citation Subset: IM |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Ascorbic Acid / pharmacology* Humans Nitrates / metabolism Nitrosamines / biosynthesis* Vitamin E / pharmacology* |
| Grant Support | |
ID/Acronym/Agency:
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1-P01-CA26731-05/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Nitrates; 0/Nitrosamines; 1406-18-4/Vitamin E; 50-81-7/Ascorbic Acid; 7519-36-0/nitrosoproline |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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