Document Detail

Effect of vitamin E on production of macrophage migration inhibitory factor (MIF) by macrophages.
MedLine Citation:
PMID:  10609875     Owner:  NLM     Status:  MEDLINE    
Macrophage migration inhibitory factor (MIF), a putative cytokine involved in inflammatory and immune responses, was identified in rat peritoneal macrophages by Western blot analysis and its secretion into culture medium by enzyme-linked immunosorbent assay. To clarify the possibility of vitamin E as an immune modulator, we investigated the effect of vitamin E on MIF production in macrophages in response to calcium ionophore A23187 and lipopolysaccharide (LPS). Intraperitoneal injections of vitamin E (5 mg per rat) for 6 successive days resulted in a significant increase of alpha-tocopherol content in peritoneal macrophages. Alpha-tocopherol content of macrophages in vitamin E-treated rats was 478.3 +/- 90.7 ng/10(6) cells, whereas in control rats it was 1.5 +/- 0.5 ng/10(6) cells. For the control macrophages, total MIF content of the medium (2.5 x 10(6) cells/18 ml) without stimulation was 40.7 +/- 3.6 ng after 14 h culture, whereas stimulation with calcium ionophore A23187 (400 nM) and LPS (5.0 microg/ml) induced the elevation of MIF content to 65.9 +/- 7.5 ng and 74.3 +/- 10.4 ng, respectively (p < 0.05, n = 3). On the other hand, vitamin E-enriched macrophages without stimulation showed less MIF content (14.0 +/- 4.2 ng) than the control (p < 0.05, n = 3). Similarly, the increase of MIF of vitamin E-treated macrophages was significantly suppressed after stimulation with calcium ionophore A23187 or LPS, compared with the control macrophages (p < 0.01, n = 3). From analysis of intracellular MIF content by Western blot, we found no alteration of intracellular MIF content of vitamin E-macrophages, in contrast to the decreased content of control stimulated-macrophages, showing that vitamin E suppressed MIF secretion into the culture medium. Taken together, these results indicate that vitamin E may contribute to the regulation of inflammatory and immune responses through regulation of MIF secretion, possibly by modulating macrophage-membrane architecture.
W Sakamoto; J Nishihira; K Fujie; T Iizuka; H Handa; M Ozaki; S Yukawa
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  BioFactors (Oxford, England)     Volume:  10     ISSN:  0951-6433     ISO Abbreviation:  Biofactors     Publication Date:  1999  
Date Detail:
Created Date:  2000-02-01     Completed Date:  2000-02-01     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8807441     Medline TA:  Biofactors     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  139-43     Citation Subset:  IM    
Department of Biochemistry, School of Dentistry, Hokkaido University, Sapporo, Japan.
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MeSH Terms
Calcimycin / pharmacology
Cell Size
Cell Survival
Cells, Cultured
Lipopolysaccharides / pharmacology
Macrophage Migration-Inhibitory Factors / biosynthesis*,  secretion
Macrophages, Peritoneal / cytology,  drug effects*,  metabolism
Rats, Wistar
Vitamin E / pharmacology*
Reg. No./Substance:
0/Lipopolysaccharides; 0/Macrophage Migration-Inhibitory Factors; 1406-18-4/Vitamin E; 52665-69-7/Calcimycin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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