Document Detail

Effect of vitamin E and eccentric exercise on selected biomarkers of oxidative stress in young and elderly men.
MedLine Citation:
PMID:  12788477     Owner:  NLM     Status:  MEDLINE    
Muscle damage resulting from eccentric exercise provides a useful model of oxyradical-induced injury and can be used to examine age-related responses to oxidative stress. Sixteen young (26.4 +/- 3.3 years) and 16 older (71.1 +/- 4.0 years) healthy men were randomly assigned to 1000 IU/d vitamin E or placebo for 12 weeks and ran downhill for 45 min at 75% VO(2)max, once before and following supplementation. Blood samples were obtained before (baseline) and immediately postexercise (0 h), and at 6, 24, and 72 h postexercise to determine antioxidant status, muscle damage, lipid peroxidation, and DNA damage. Following exercise, young and older men experienced similar increases in serum creatine kinase (CK), F(2alpha)-isoprostanes (iPF(2alpha); p <.001) and malondialdehyde (MDA; p <.01), although iPF(2alpha) peaked at 72 h postexercise and MDA peaked at 0 h. Oxygen Radical Absorbance Capacity (ORAC) decreased at 72 h (p <.01) and correlated with the rise in iPF(2alpha), MDA, and CK in the young men (p <.05). Leukocyte 8-hydroxy-2'-deoxyguanosine (8-OHdG) was unaffected by exercise. Vitamin E decreased peak CK in young men, while in older men it decreased resting levels of iPF(2alpha) and suppressed the 24 h postexercise increases in iPF(2alpha) (p <.05). Thus, vitamin E supplementation induced modest changes eccentric exercise-induced oxidative stress, although differentially between the young and older subjects, while age had no direct influence on these responses among this group of physically fit subjects.
Jennifer M Sacheck; Paul E Milbury; Joseph G Cannon; Ronenn Roubenoff; Jeffrey B Blumberg
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Publication Detail:
Type:  Clinical Trial; Journal Article; Randomized Controlled Trial; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Free radical biology & medicine     Volume:  34     ISSN:  0891-5849     ISO Abbreviation:  Free Radic. Biol. Med.     Publication Date:  2003 Jun 
Date Detail:
Created Date:  2003-06-05     Completed Date:  2004-02-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8709159     Medline TA:  Free Radic Biol Med     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1575-88     Citation Subset:  IM    
Nutrition, Exercise Physiology, and Sarcopenia Laboratory, Tufts University, Boston, MA 02111, USA.
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MeSH Terms
Aged, 80 and over
Aging / physiology*
Antioxidants / pharmacology*
Biological Markers / analysis*
Creatine Kinase / blood
Deoxyguanosine / analogs & derivatives*,  metabolism
Dietary Supplements
Double-Blind Method
F2-Isoprostanes / metabolism
Lipid Peroxidation
Malondialdehyde / metabolism
Muscle, Skeletal / metabolism
Oxidative Stress / drug effects*
Vitamin E / pharmacology*
Reg. No./Substance:
0/8-hydroxy-2'-deoxyguanosine; 0/Antioxidants; 0/Biological Markers; 0/F2-Isoprostanes; 1406-18-4/Vitamin E; 542-78-9/Malondialdehyde; 961-07-9/Deoxyguanosine; EC Kinase

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