Document Detail


Effect of virgin olive oil plus acetylsalicylic acid on brain slices damage after hypoxia-reoxygenation in rats with type 1-like diabetes mellitus.
MedLine Citation:
PMID:  20079805     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Aspirin is the most widely used drug for the secondary prevention of ischemic stroke in patients suffering from diabetes mellitus. Moreover virgin olive oil (VOO) administration exerts a neuroprotective effect in healthy rat brain slices. The aim of the present study was to determine the possible influence of VOO administration to streptozotocin-diabetic rats (DR) on the neuroprotective effect of aspirin in rat brain. DR were treated during 3 months with saline, aspirin (2mg/kg/day p.o.), VOO (0.5 mL/kg/day p.o.) or its association; a control normoglycemic group was treated with saline. Brain slices were subjected to oxygen-glucose deprivation before a reoxygenation period. All the treatments significantly reduced lactate dehydrogenase LDH efflux after reoxygenation (-54.1% for aspirin, -51.3% for VOO and -72.9% for aspirin plus VOO). Lipid peroxides in brain slices were also reduced after the treatment with aspirin (-17.90%), VOO (-37.3%) and aspirin plus VOO (-49.2%). Production of nitric oxide after reoxygenation was inhibited by all the treatments (-46.5% for ASA, -48.2% for VOO and -75.8% for ASA plus VOO). The activity of the inducible isoform (iNOS) was inhibited by the three types of treatment (-31.8% for ASA, -29.1% for VOO and -56.0% for ASA plus VOO). The main conclusion of our study is that daily oral administration of VOO to diabetic rats may be a natural way to increase the neuroprotective effect of aspirin in diabetic animals.
Authors:
Jos? Pedro De La Cruz; Sergio Del R?o; Mar?a Monsalud Arrebola; Juan Antonio L?pez-Villodres; Nuria Jebrouni; Jos? Antonio Gonz?lez-Correa
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-01-15
Journal Detail:
Title:  Neuroscience letters     Volume:  471     ISSN:  1872-7972     ISO Abbreviation:  Neurosci. Lett.     Publication Date:  2010 Mar 
Date Detail:
Created Date:  2010-02-17     Completed Date:  2010-04-16     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7600130     Medline TA:  Neurosci Lett     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  89-93     Citation Subset:  IM    
Copyright Information:
(c) 2010 Elsevier Ireland Ltd. All rights reserved.
Affiliation:
Laboratorio de Investigaciones Antitromb?ticas e Isquemia Tisular (LIAIT), Department of Pharmacology and Therapeutics, School of Medicine, University of M?laga, Spain.
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MeSH Terms
Descriptor/Qualifier:
Animals
Aspirin / pharmacology*
Brain / drug effects*,  metabolism
Diabetes Mellitus, Experimental / complications
Diabetes Mellitus, Type 1 / complications*
Hypoxia, Brain / etiology,  metabolism,  prevention & control*
L-Lactate Dehydrogenase / metabolism
Lipid Peroxidation
Male
Neuroprotective Agents / pharmacology*
Nitric Oxide / biosynthesis
Nitric Oxide Synthase Type II / antagonists & inhibitors
Plant Oils / pharmacology*
Rats
Rats, Wistar
Chemical
Reg. No./Substance:
0/Neuroprotective Agents; 0/Plant Oils; 10102-43-9/Nitric Oxide; 50-78-2/Aspirin; 8001-25-0/olive oil; EC 1.1.1.27/L-Lactate Dehydrogenase; EC 1.14.13.39/Nitric Oxide Synthase Type II

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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