Document Detail

Effect of verapamil and sulfhydryl reagents on calcium transport in bovine spermatozoa.
MedLine Citation:
PMID:  3593837     Owner:  NLM     Status:  MEDLINE    
Calcium uptake by intact bovine epididymal spermatozoa is not affected by low concentrations (up to 0.75 mM) of the calcium transport blocker verapamil. Under these conditions, calcium transport into sperm mitochondria is highly inhibited. At higher verapamil concentrations (1.0, 1.5 mM), calcium transport into intact sperm is also inhibited, and this inhibition cannot be relieved by disrupting the plasma membrane with filipin. Calcium uptake into intact sperm is highly inhibited by mersalyl and this inhibitory effect can be completely relieved when the plasma membrane is disrupted by filipin. This effect of mersalyl is not dependent on the presence of phosphate in the incubation medium. Phosphate itself, up to 2 mM, enhances calcium uptake into the cells; this effect decreases at higher concentrations and is depressed 57% at 10 mM phosphate. This inhibitory effect of high phosphate concentration can be blocked by mersalyl. It is suggested that the calcium carrier itself and not a phosphate carrier of the plasma membrane is inhibited by mersalyl. It is possible that there is a symporter for calcium and phosphate in the plasma membrane of bovine spermatozoa.
H Breitbart; H A Lardy
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biology of reproduction     Volume:  36     ISSN:  0006-3363     ISO Abbreviation:  Biol. Reprod.     Publication Date:  1987 Apr 
Date Detail:
Created Date:  1987-07-24     Completed Date:  1987-07-24     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0207224     Medline TA:  Biol Reprod     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  658-63     Citation Subset:  IM    
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MeSH Terms
Biological Transport, Active / drug effects
Calcium / metabolism*
Cell Membrane / drug effects,  metabolism
Ethylmaleimide / pharmacology
Mersalyl / pharmacology
Mitochondria / drug effects,  metabolism
Spermatozoa / drug effects*,  metabolism
Sulfhydryl Reagents / pharmacology*
Verapamil / pharmacology*
Grant Support
Reg. No./Substance:
0/Sulfhydryl Reagents; 128-53-0/Ethylmaleimide; 492-18-2/Mersalyl; 52-53-9/Verapamil; 7440-70-2/Calcium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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