| Effect of verapamil and nifedipine on cholesteryl ester metabolism and low-density lipoprotein oxidation in macrophages. | |
MedLine Citation:
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PMID: 7857326 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Using mouse macrophage cultures, the effects of verapamil and nifedipine on cholesteryl ester and low-density lipoprotein (LDL) metabolism were studied with special reference to the following parameters: (a) incorporation of [14C]oleate into cholesteryl esters (ChE), (b) contents of total and free cholesterol (FCh), (c) liberation of [14C]oleate from ChE and incorporation of [3H]FCh into ChE, (d) excretion of [3H]Ch from the cells, and (e) LDL oxidation. Verapamil and nifedipine (10-100 microM) were shown to decrease in a dose-dependent manner the incorporation of [14C]oleate into ChE and to increase the concentration of FCh but had no appreciable effect on the concentration of total cholesterol in macrophages cultured in the presence of acetylated LDL. The drugs stimulated the liberation of [14C]oleate from cellular ChE. The pharmacological concentrations (25-75 microM) of verapamil and nifedipine increased the excretion of [3H]FCh from ChE of macrophages in the presence of serum and high-density lipoproteins. The same concentrations of the drugs inhibited both LDL-derived malonyldialdehyde-like products and nitroblue tetrazolium dye reduction in a dose-dependent fashion. The results obtained suggest that verapamil and nifedipine exert their macrophage-mediated antiatherosclerotic effect via reduction of LDL oxidative modification, reduction of intracellular ChE synthesis, stimulation of ChE hydrolysis and cholesterol excretion from the cells. |
Authors:
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M I Dushkin; Y S Schwartz |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Biochemical pharmacology Volume: 49 ISSN: 0006-2952 ISO Abbreviation: Biochem. Pharmacol. Publication Date: 1995 Jan |
Date Detail:
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Created Date: 1995-03-14 Completed Date: 1995-03-14 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0101032 Medline TA: Biochem Pharmacol Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 389-97 Citation Subset: IM |
Affiliation:
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Department of Atherogenesis, Siberian Branch of Russian Academy of Medical Science, Novosibirsk. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cells, Cultured Cholesterol / analysis Cholesterol Esters / metabolism* Esterification / drug effects Lipoproteins, LDL / metabolism* Macrophages / drug effects*, metabolism Mice Nifedipine / pharmacology* Oleic Acid Oleic Acids / metabolism Oxidation-Reduction / drug effects Tetrazolium Salts Verapamil / pharmacology* |
| Chemical | |
Reg. No./Substance:
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0/Cholesterol Esters; 0/Lipoproteins, LDL; 0/Oleic Acids; 0/Tetrazolium Salts; 112-80-1/Oleic Acid; 1871-22-3/4-anisyltetrazolium blue; 21829-25-4/Nifedipine; 52-53-9/Verapamil; 57-88-5/Cholesterol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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