Document Detail

Effect of varying freezing and thawing rates in experimental cryosurgery.
MedLine Citation:
PMID:  3979086     Owner:  NLM     Status:  MEDLINE    
Six different freezing/thawing programs, which varied freezing rate, duration of freezing, and thawing rates, were used to investigate the effect of these factors on cell destruction in dog skin. The range of tissue temperatures produced was from -15 to -50 degrees C. The extent of destruction was evaluated by skin biopsies 3 days after cold injury. In single, short freezing/thawing cycles, the temperature reached in the tissue was the prime factor in cell death. Longer freezing time and slow thawing were also important lethal factors which increased destruction of cells. Cooling rate, whether slow or fast, made little difference in the outcome. The experiments suggested that present-day, commonly employed cryosurgical techniques, which feature fast cooling, slow thawing, and repetition of the freeze/thaw cycle, should be modified by the use of maintenance of the tissue in the frozen state for several minutes and slow thawing. Thawing should be complete before freezing is repeated. These modifications in technique will maximize tissue destruction, an important consideration in cancer cryosurgery.
A A Gage; K Guest; M Montes; J A Caruana; D A Whalen
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cryobiology     Volume:  22     ISSN:  0011-2240     ISO Abbreviation:  Cryobiology     Publication Date:  1985 Apr 
Date Detail:
Created Date:  1985-05-20     Completed Date:  1985-05-20     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0006252     Medline TA:  Cryobiology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  175-82     Citation Subset:  IM    
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MeSH Terms
Hot Temperature
Skin / surgery*
Time Factors

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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