Document Detail


Effect of various concentrations of acyclovir on cell survival and micronuclei induction on cultured HeLa cells.
MedLine Citation:
PMID:  10635337     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The HeLa cells were treated with 0, 0.01, 0.1, 1, 10 and 100 microM acyclovir (ACV) for 8 h duration and the growth kinetics, cell survival and micronuclei induction were determined. Treatment of HeLa cells with various concentrations of ACV resulted in a concentration-dependent decline in growth kinetics, cell proliferation indices and cell survival. ACV, 100 microM, completely inhibited cell division, where no appreciable changes in cell number were observed from 1 to 5 days post-treatment. This is reflected in cell survival, where the surviving fraction of cells was reduced to 1/2 at 100 microM ACV. Conversely, the frequency of micronuclei showed a concentration-dependent elevation at 20, 30 and 40 h post-treatment. ACV not only induced one micronuclei-bearing binucleate cell but also binucleate cells bearing two and multiple micronuclei in a concentration-dependent manner. The micronuclei frequency increased with time up to 30 h post-treatment and declined thereafter. The relationship between micronuclei induction and cell survival was determined by plotting the former on Y- and the latter on X-axes, respectively. The surviving fraction of cells declined with the elevation in micronuclei frequency and a best fit was observed for linear quadratic formalism.
Authors:
G C Jagetia; R Aruna
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Mutation research     Volume:  446     ISSN:  0027-5107     ISO Abbreviation:  Mutat. Res.     Publication Date:  1999 Dec 
Date Detail:
Created Date:  2000-02-10     Completed Date:  2000-02-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0400763     Medline TA:  Mutat Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  155-65     Citation Subset:  IM    
Affiliation:
Department of Radiobiology, Kasturba Medical College, Manipal, India. info@kmc.ernet.in
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MeSH Terms
Descriptor/Qualifier:
Acyclovir / administration & dosage,  pharmacology*
Antiviral Agents / administration & dosage,  pharmacology*
Cell Division / drug effects
Cell Nucleus / drug effects,  genetics*
Cell Survival / drug effects*,  genetics
Dose-Response Relationship, Drug
Hela Cells / drug effects
Humans
Micronucleus Tests
Tumor Stem Cell Assay / methods
Chemical
Reg. No./Substance:
0/Antiviral Agents; 59277-89-3/Acyclovir

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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