| Effect of ursodeoxycholic acid on inflammatory infiltrate in gallbladder muscle of cholesterol gallstone patients. | |
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MedLine Citation:
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PMID: 20426797 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Reduced gallbladder (GB) contractility and chronic inflammatory changes in the mucosa have been reported in patients with cholesterol gallstones (GS). Ursodeoxycholic acid (UDCA) restores GB contractility and antagonises liver macrophage activation. In the colon, hydrophobic bile acid, not hydrophilic UDCA, induces mast cell degranulation. We studied the presence of monocyte/macrophage infiltrate, cyclooxygenase (COX)-2, inducible nitric oxide synthase (iNOS) expression, the number of total and degranulated mast cells in the GB muscle layer of cholesterol GS patients, and the effect of UDCA administration. METHODS: Gallbladder tissue was obtained from cholesterol GS patients, either treated or untreated with UDCA (10 mg kg(-1) day(-1)) for 30 days prior to surgery. Gallbladders removed for neoplastic diseases, not involving GB, were evaluated for control purposes. The presence of monocytes/macrophages (CD68 positive), granulocytes, and mast cells, and the COX-2 and iNOS expression, was determined immunohistochemically. KEY RESULTS: The number of CD68, granulocytes, mast cells, COX-2 and iNOS positive cells was significantly higher in the muscle layer of GS patients than in controls. Compared to untreated patients, those treated with UDCA showed significantly lower levels of CD68, COX-2 positive cells and degranulated mast cells and a lesser number of iNOS positive cells and granulocytes. CONCLUSIONS & INFERENCES: An inflammatory monocyte/macrophage, mast cell and granulocyte infiltrate is present in the GB muscle layer of GS patients. Ursodeoxycholic acid decreases macrophages, degranulated mast cells and COX-2 expression. These results suggest that monocytes/macrophages and degranulating mast cells contribute to muscle cell dysfunction in cholesterol GS patients and support the anti-inflammatory effect of UDCA. |
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Authors:
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S Carotti; M P L Guarino; M Cicala; G Perrone; R Alloni; F Segreto; C Rabitti; S Morini |
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Publication Detail:
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Type: Journal Article; Randomized Controlled Trial Date: 2010-04-20 |
Journal Detail:
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Title: Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society Volume: 22 ISSN: 1365-2982 ISO Abbreviation: Neurogastroenterol. Motil. Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-07-28 Completed Date: 2010-11-22 Revised Date: 2011-10-27 |
Medline Journal Info:
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Nlm Unique ID: 9432572 Medline TA: Neurogastroenterol Motil Country: England |
Other Details:
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Languages: eng Pagination: 866-73, e232 Citation Subset: IM |
Affiliation:
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Department of Biomedical Research (CIR), University Campus Bio-Medico, Rome, Italy. |
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Aged Aged, 80 and over Antigens, CD / metabolism Antigens, Differentiation, Myelomonocytic / metabolism Cholagogues and Choleretics / pharmacology* Cholesterol / chemistry* Cyclooxygenase 2 / metabolism Gallbladder* / anatomy & histology, drug effects, pathology Gallstones / chemistry* Granulocytes / metabolism Humans Inflammation / pathology Male Mast Cells / metabolism Middle Aged Nitric Oxide Synthase Type II / metabolism Ursodeoxycholic Acid / pharmacology* |
| Chemical | |
Reg. No./Substance:
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0/Antigens, CD; 0/Antigens, Differentiation, Myelomonocytic; 0/CD68 antigen, human; 0/Cholagogues and Choleretics; 128-13-2/Ursodeoxycholic Acid; 57-88-5/Cholesterol; EC 1.14.13.39/NOS2 protein, human; EC 1.14.13.39/Nitric Oxide Synthase Type II; EC 1.14.99.1/Cyclooxygenase 2; EC 1.14.99.1/PTGS2 protein, human |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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