| Effect of uremia on aldosterone metabolism in the isolated perfused rat liver. | |
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MedLine Citation:
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PMID: 8487670 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The effect of uremia on hepatic metabolism of aldosterone was studied in the isolated perfused liver of female Wistar rats. Uremia was induced by five-sixths partial nephrectomy 4 weeks before experiments. Isolated livers of normal and uremic rats were perfused at a constant flow rate with a hemoglobin-free medium, to which 4-14C-D-aldosterone was added at 3 nmol/L. Aldosterone was analyzed by radioimmunoassay (RIA) and 4-14C-D-aldosterone radiometabolites in perfusate and bile were assayed by high-performance liquid chromatography (HPLC). Uremic rats had a 10% lower body weight (P < .01) and increased plasma urea, creatinine, and parathyroid hormone (PTH) levels (258%, 200%, and 208%, respectively; P < .01-.001). Blood pressure and plasma K+, Na+, and aldosterone levels were similar. Plasma renin activity was suppressed by 68% in uremic rats (P < .001). Liver wet weight and hepatic function were similar in livers of both groups of rats. Hepatic elimination of aldosterone was compatible with a first-order kinetics. Hepatic clearance of aldosterone per liver and per gram liver was similar; however, when expressed per 100 g rat body weight, a 21% higher value was observed in uremic rats (11.6 +/- 1.8 mL/min) compared with normal rats (9.6 +/- 1.5 mL/min, P < .01). Polar aldosterone radiometabolites accumulated in the perfusate to approximately 40% of the initial 14C added at 15 minutes, and were eliminated in bile at a similar rate in both groups. No qualitative difference was found in the pattern of radiometabolites of aldosterone in perfusate and bile.(ABSTRACT TRUNCATED AT 250 WORDS) |
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Authors:
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M Egfjord; H Daugaard; K Olgaard |
Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Metabolism: clinical and experimental Volume: 42 ISSN: 0026-0495 ISO Abbreviation: Metab. Clin. Exp. Publication Date: 1993 Apr |
Date Detail:
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Created Date: 1993-06-04 Completed Date: 1993-06-04 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0375267 Medline TA: Metabolism Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 470-6 Citation Subset: IM |
Affiliation:
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Medical Department P, Rigshospitalet, University of Copenhagen, Denmark. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aldosterone
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analogs & derivatives,
metabolism* Animals Bile / metabolism Body Weight Chromatography, High Pressure Liquid Creatinine / blood Female Kinetics Liver / metabolism* Nephrectomy Parathyroid Hormone / blood Radioimmunoassay Rats Rats, Wistar Renin / blood Urea / blood Uremia / etiology, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Parathyroid Hormone; 13489-75-3/tetrahydroaldosterone; 52-39-1/Aldosterone; 57-13-6/Urea; 60-27-5/Creatinine; EC 3.4.23.15/Renin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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