Document Detail


Effect of unsaturated fatty acids on sterol biosynthesis in yeast.
MedLine Citation:
PMID:  7016186     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Lipid-depleted yeast, grown anaerobically, contains only very low amounts of sterols. The hydroxymethylglutaryl-CoA reductase activity, the regulatory enzyme of sterol synthesis in yeast, is also low. Aeration of such cells in a buffer containing a carbon source induces hydroxymethylglutaryl-CoA reductase activity and increases sterol synthesis. The velocity of the increase depends on the carbon source present during the aeration period. Glucose and sugars that are easily converted to glucose were found to be most effective. A supplement of unsaturated fatty acids during anaerobic growth causes a several-fold greater velocity of the enzyme induction and of sterol biosynthesis. Linolenic acid (30 microM) accelerated sterol biosynthesis about 7-fold. Activities of galactokinase and galactose-1-phosphate uridyltransferase, which are involved in the conversion of galactose to glucose, increased several-fold in the supplemented cells within 6 h of aeration, concomitantly with stimulation of sterol synthesis from galactose. It is suggested that the stimulation of enzyme induction and sterol biosynthesis in fatty acid supplemented cells is due to a completion of the protein-synthesizing apparatus during cell growth. A markedly enhanced capacity of these cells to incorporate leucine into acid-precipitable protein supports this assumption.
Authors:
M Boll; M Löwel; J Berndt
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  620     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1980 Dec 
Date Detail:
Created Date:  1981-08-10     Completed Date:  1981-08-10     Revised Date:  2000-12-18    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  429-39     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Acetates / pharmacology
Aerobiosis
Enzyme Induction / drug effects
Fatty Acids, Unsaturated / pharmacology*
Glucose / pharmacology
Hydroxymethylglutaryl CoA Reductases / biosynthesis
Saccharomyces cerevisiae / metabolism*
Sterols / biosynthesis*
Stimulation, Chemical
Time Factors
Chemical
Reg. No./Substance:
0/Acetates; 0/Fatty Acids, Unsaturated; 0/Sterols; 50-99-7/Glucose; EC 1.1.1.-/Hydroxymethylglutaryl CoA Reductases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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