Document Detail


Effect of two novel CGRP-binding compounds in a closed cranial window rat model.
MedLine Citation:
PMID:  17477918     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We investigated the in vivo effects of two novel calcitonin gene-related peptide (CGRP) binding molecules in the genuine closed cranial window model in the rat. The RNA-Spiegelmer (NOX-C89) and the monoclonal CGRP antibody are CGRP scavengers and might be used as an alternative to CGRP-receptor antagonists in the treatment of migraine. Rats were anaesthetized and a closed cranial window established. Changes in dural and pial artery diameter and mean arterial blood pressure were measured simultaneously. Infusion of the RNA-Spiegelmer or the CGRP antibody alone had no effect on the arteries or the mean arterial blood pressure. We then used a bolus of 0.3 microg/kg CGRP (n=6) or electrical stimulation (25 V, 5 Hz, 1 ms pulse width and of 10 s of duration) (n=6) to induce dilatation of dural and pial arteries (mediated via CGRP-receptors). Pre-treatment with the RNA-Spiegelmer inhibited CGRP-induced vasodilatation of the dural artery (from 38+/-17% to 7+/-3%) and the pial artery (from 14+/-1% to 3+/-2%) (P<0.05). The RNA-Spiegelmer, however, did not significantly inhibit dilatation induced by electrical stimulation (P>0.05). The CGRP antibody caused a significant reduction of the dural artery diameter caused by intravenous CGRP-infusion (from 23+/-5% to 12+/-3%) (P<0.05), but did not inhibit dilatation caused by electrical stimulation (P>0.05). In conclusion, the CGRP scavengers effectively inhibited the effect of circulating CGRP but do not modify the effect of electrical stimulation and the consequent liberation of CGRP from perivascular sensory nerve fibres.
Authors:
Louise Juhl; Lars Edvinsson; Jes Olesen; Inger Jansen-Olesen
Related Documents :
22955728 - Dealcoholized red wine decreases systolic and diastolic blood pressure and increases pl...
12492778 - Central osmotic regulation of sympathetic nerve activity.
24493928 - Effect of eprosartan-based antihypertensive therapy on coronary heart disease risk asse...
25091358 - Association between control to target blood pressures and healthy lifestyle factors amo...
24469698 - Measurement of a gradient of oxygen partial pressure across the intact root.
20836698 - Lentiviral-human heme oxygenase targeting endothelium improved vascular function in ang...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-04-14
Journal Detail:
Title:  European journal of pharmacology     Volume:  567     ISSN:  0014-2999     ISO Abbreviation:  Eur. J. Pharmacol.     Publication Date:  2007 Jul 
Date Detail:
Created Date:  2007-05-28     Completed Date:  2007-07-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  1254354     Medline TA:  Eur J Pharmacol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  117-24     Citation Subset:  IM    
Affiliation:
Department of Neurology, Glostrup Research Institute, Glostrup Hospital, University of Copenhagen, DK-2600, Glostrup, Denmark. loujuh01@glo.regionh.dk
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Monoclonal / pharmacology*
Aptamers, Nucleotide / pharmacology*
Blood Pressure / drug effects
Calcitonin Gene-Related Peptide / immunology,  metabolism*,  pharmacology
Disease Models, Animal
Dura Mater / blood supply
Electric Stimulation
Male
Meningeal Arteries / drug effects,  physiopathology
Middle Cerebral Artery / drug effects,  physiopathology
Migraine Disorders / drug therapy
Oligonucleotides / pharmacology*
Pia Mater / blood supply
Polyethylene Glycols / chemistry
Protein Binding
Rats
Rats, Sprague-Dawley
Vasodilation / drug effects
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Aptamers, Nucleotide; 0/NOX-C29; 0/Oligonucleotides; 0/Polyethylene Glycols; 83652-28-2/Calcitonin Gene-Related Peptide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Protective effect of Umbelliferone on membranous fatty acid composition in streptozotocin-induced di...
Next Document:  Comparison of the effects of bupropion on nicotinic receptor-evoked [(3)H]dopamine release from rat ...