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Effect of treadmill running on the expression of genes that are involved in neuronal differentiation in the hippocampus of adult male rats.
MedLine Citation:
PMID:  23427087     Owner:  NLM     Status:  Publisher    
Gene expression in the rodent brain is dependent on exercise. However, our findings regarding this fact are inadequate. The aim of this study was to evaluate the effect of exercise intensity on changes in factors involved in neuronal differentiation and plasticity in the hippocampus of adult male rats. Thus, the effects of 2 weeks treadmill running at of low (11 m/min) and high (25 m/min) intensities, 30 min/day for 14 consecutive days on the expression of two genes, microRNA-124 and neuron-restrictive silencer factor/RE1-silencing transcription factor (REST) as well as changes of two proteins of brain-derived neurotrophic factor (BDNF) and tyrosine kinase B (TrkB) were analysed. Using quantitative real-time PCR techniques have shown that both exercise intensities reduced the expression of REST (31% in low exercise intensity, 52% in high exercise intensity) and elevated the expression of miR-124 (3.8-fold in low exercise intensity, 11.9-fold in high exercise intensity) significantly (P ≤ 0.05). This suggests that exercise probably through the changes in these genes is involved in neuronal differentiation in the hippocampus of rats. BDNF and TrkB levels were measured by ELISA. Exercise, at low intensity, increased TrkB (4.51 ng/L vs. 3.73 ng/L) and BDNF (11.97 ng/L vs. 11.08 ng/L) proteins significantly (P ≤ 0.05), while at high intensity, the changes in comparison with the control group were not significant. Expression levels during the exercise programme with high intensity were dramatic for miR-124 and less dramatic for REST compared with low intensity exercise programme.
Shima Mojtahedi; Mohammad Reza Kordi; Seyed Ebrahim Hosseini; Simin Fallah Omran; Masoud Soleimani
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-12-17
Journal Detail:
Title:  Cell biology international     Volume:  -     ISSN:  1095-8355     ISO Abbreviation:  Cell Biol. Int.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2013-2-21     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9307129     Medline TA:  Cell Biol Int     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2013 International Federation for Cell Biology.
Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Tehran, Tehran, Iran.
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