| Effect of transport at ambient temperature on detection and isolation of Vibrio cholerae from environmental samples. | |
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MedLine Citation:
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PMID: 16517670 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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It has long been assumed that prolonged holding of environmental samples at the ambient air temperature prior to bacteriological analysis is detrimental to isolation and detection of Vibrio cholerae, the causative agent of pandemic cholera. The present study was aimed at understanding the effect of transporting environmental samples at the ambient air temperature on isolation and enumeration of V. cholerae. For water and plankton samples held at ambient temperatures ranging from 31 degrees C to 35 degrees C for 20 h, the total counts did not increase significantly but the number of culturable V. cholerae increased significantly compared to samples processed within 1 h of collection, as measured by culture, acridine orange direct count, direct fluorescent-antibody-direct viable count (DFA-DVC), and multiplex PCR analyses. For total coliform counts, total bacterial counts, and DFA-DVC counts, the numbers did not increase significantly, but the culturable plate counts for V. cholerae increased significantly after samples were held at the ambient temperature during transport to the laboratory for analysis. An increase in the recovery of V. cholerae O1 and improved detection of V. cholerae O1 rfb and ctxA also occurred when samples were enriched after they were kept for 20 h at the ambient temperature during transport. Improved detection and isolation of toxigenic V. cholerae from freshwater ecosystems can be achieved by holding samples at the ambient temperature, an observation that has significant implications for tracking this pathogen in diverse aquatic environments. |
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Authors:
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Munirul Alam; Abdus Sadique; Nur-A-Hasan; Nurul A Bhuiyan; G Balakrish Nair; A K Siddique; David A Sack; Sunjukta Ahsan; Anwar Huq; R Bradley Sack; Rita R Colwell |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Applied and environmental microbiology Volume: 72 ISSN: 0099-2240 ISO Abbreviation: Appl. Environ. Microbiol. Publication Date: 2006 Mar |
Date Detail:
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Created Date: 2006-03-06 Completed Date: 2006-05-30 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 7605801 Medline TA: Appl Environ Microbiol Country: United States |
Other Details:
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Languages: eng Pagination: 2185-90 Citation Subset: IM |
Affiliation:
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International Center for Diarrhoeal Disease Research, Dhaka, Bangladesh. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bacterial Proteins
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genetics Colony Count, Microbial Environmental Monitoring / methods* Fresh Water / microbiology* Plankton / chemistry Polymerase Chain Reaction / methods Temperature* Vibrio cholerae / classification, genetics, growth & development*, isolation & purification* |
| Grant Support | |
ID/Acronym/Agency:
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1R01A13912901//PHS HHS |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Proteins |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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