Document Detail

Effect of transient ischemia on long-term potentiation of synaptic transmission in rat hippocampal slices.
MedLine Citation:
PMID:  18987602     Owner:  NLM     Status:  MEDLINE    
OBJECTIVES: Long-term potentiation (LTP) of neuronal activity in the hippocampus is thought to be a substrate for learning and memory. The influence of ischemia (IS) (hypoxia/hypoglycemia) on induction of LTP of synaptic transmission (ST) by high frequency stimulation (HFS) was investigated in rat hippocampal slices. METHODS: Neurons were stimulated via Schäffer collaterals and field excitatory postsynaptic potentials (fEPSP) were recorded extracellularly from the CA1 region. LTP was induced by one single train (100 Hz, 1s). RESULTS: In controls LTP of ST after HFS was 179.70+/-12.53%. Short IS of 2.5-4.5 min elicited a transient failure of ST, with return to former value followed by further increase of fEPSP amplitude to 142.28+/-16.24%, compared to amplitude before IS. HFS was elicited 40 min after exposure to IS and LTP was measured over further 40-60 min. LTP in slices exposed to 2.5-4.5-min IS was 139.94+/-14.01%. IS of 6-7.5 min elicited a prolonged failure of ST, with almost full recovery (96.69+/-14.42%). LTP was not activated 40 min after 6-7.5-min IS and the amplitude of fEPSP was even reduced to 80.14+/-19.19% compared to the former mean value of fEPSP 10 min before HFS. CONCLUSION: The results revealed that prolonged 6-7.5-min IS influenced induction of LTP of ST in the hippocampus and thus it could have deleterious effects on learning and memory. These findings may have clinical implications in stroke, brain ischemia, sleep apnoe and call for studying the effect of neuroprotectants on the induction of LTP in hippocampus exposed to oxidative stress.
Zdenka Gasparova; Pavol Jariabka; Svorad Stolc
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Neuro endocrinology letters     Volume:  29     ISSN:  0172-780X     ISO Abbreviation:  Neuro Endocrinol. Lett.     Publication Date:  2008 Oct 
Date Detail:
Created Date:  2008-11-25     Completed Date:  2009-02-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8008373     Medline TA:  Neuro Endocrinol Lett     Country:  Sweden    
Other Details:
Languages:  eng     Pagination:  702-5     Citation Subset:  IM    
Institute of Experimental Pharmacology, Slovak Academy of Sciences, Bratislava, Slovakia.
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MeSH Terms
Electric Stimulation
Excitatory Postsynaptic Potentials / physiology
Hippocampus / blood supply*,  physiology*
Ischemic Attack, Transient / physiopathology*
Long-Term Potentiation / physiology*
Rats, Wistar
Synaptic Transmission / physiology*

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