Document Detail


Effect of trans-10 cis-12 conjugated linoleic acid on Bovine Oocyte Competence and Fatty Acid Composition.
MedLine Citation:
PMID:  21366717     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The reproductive performance of dairy cows may be improved by feeding conjugated linoleic acid (CLA) supplements during early lactation. The mechanism of action of t10,c12 CLA is not clearly known. Our objective was to investigate the effect of t10,c12 CLA on oocyte maturation and lipid composition of cumulus oocyte complexes (COC). The developmental potential of oocytes incubated in in vitro maturation (IVM) medium supplemented with t10,c12 CLA to the blastocyst stage and embryo quality were also assessed. In experiment 1, abattoir-derived oocytes were matured in TCM199 + 10% serum supplemented with 100 μM t10,c12 CLA (t10,c12 CLA n = 672) or without it (control n = 672). Mature oocytes were either stained for chromatin configuration or inseminated and cultured for embryo development assessment. In experiment 2, COC and IVM culture media were subjected to fatty acid (FA) analysis prior and after maturation with t10,c12 CLA or without it (control). Total lipids and FA profiles in oocytes, cumulus cells and culture media were determined by gas chromatography. t10,c12 CLA supplementation to IVM medium improved (p = 0.05) embryo quality evaluated morphologically. This effect was associated with t10,c12 CLA presence (3.1 ± 0.7%, p = 0.04) and lower levels of arachidonic acid in FA profile of t10,c12 CLA mature oocytes (immature oocytes = 4.4 ± 1.9%, t10,c12 CLA mature oocytes = 1.0 ± 0.7%, p = 0.05). Differences in myristic and eicotrienoic acids, saturated and unsaturated FA concentrations between oocytes and cumulus cells were detected (p ≤ 0.05). In conclusion, the presence of t10,c12 CLA during maturation interfered on lipid metabolism improving bovine oocyte competence to develop into higher quality embryos.
Authors:
M Lapa; Cc Marques; Sp Alves; Mi Vasques; Mc Baptista; I Carvalhais; M Silva Pereira; Aem Horta; Rjb Bessa; Rm Pereira
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-3-1
Journal Detail:
Title:  Reproduction in domestic animals = Zuchthygiene     Volume:  -     ISSN:  1439-0531     ISO Abbreviation:  -     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-3-3     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9015668     Medline TA:  Reprod Domest Anim     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2011 Blackwell Verlag GmbH.
Affiliation:
L-INIA Santarém, Instituto Nacional dos Recursos Biológicos, Quinta da Fonte Boa, Vale de Santarém, Portugal REQUIMTE, ICBAS, Universidade do Porto, Vairão, Portugal Departamento de Ciências Agrárias, Universidade dos Açores, Angra do Heroísmo Açores, Portugal Centro de Investigação Interdisciplinar em Sanidade Animal, FMV - UTL, Lisboa, Portugal Escola Universitária Vasco da Gama, Mosteiro de S. Jorge de Milreu, Coimbra, Portugal.
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