Document Detail


Effect of topoisomerase II inhibitors on hyperthermic cytotoxicity.
MedLine Citation:
PMID:  2838165     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Exposure of HeLa or Chinese hamster ovary cells to drugs (novobiocin, nalidixic acid, or oxolinic acid) which inhibit the nuclear enzyme topoisomerase II resulted in a sensitization of both cell lines to hyperthermic heating at 41 and 45 degrees C. Exposure to 0.5 mg/ml novobiocin decreased the reciprocal slope (T0) of the survival curve of HeLa cells heated at 41 and 45 degrees C by a factor of 7.5 and 2.4, respectively. Exposure to 0.5 mg/ml novobiocin decreased the T0 of the survival curve of Chinese hamster ovary cells heated at 41 and 45 degrees C by a factor of 9.8 and 1.8, respectively. Exposure of HeLa cells to 0.5 mg/ml novobiocin delayed thermotolerance development for 1.5 h and depressed by a factor of 27 the survival of cells heated at 45 degrees C once thermotolerance had developed. Coincident with the sensitization to heat-induced cytotoxicity, an enhancement of a heat-induced increase in the total protein mass co-isolating with the nuclei or nuclear matrices from heated cells was observed. A log-linear correlation was found between the reduction in cell survival and the relative nuclear matrix protein mass increase in cells heated at 41 or 45 degrees C in the presence or absence of these drugs. The results are consistent with the notion that exposure to these drugs disrupts the cell's capacity to regulate nuclear structure and composition, and thus enhances heat-induced cytotoxicity.
Authors:
R L Warters; L M Brizgys
Related Documents :
15179585 - Clinal variation in body and cell size in a widely distributed vertebrate ectotherm.
2188365 - Hsp104 required for induced thermotolerance.
11999425 - Temperature-dependent production of carotovoricin er and pectin lyase in phytopathogeni...
11368525 - Differential degradation of the three fibrinogen chains by proteasomes: involvement of ...
6271915 - Simian virus 40 causes persistent infection of muntjac cells in the absence of virus tr...
23751075 - Distinguishing folate-receptor-positive cells from folate-receptor-negative cells by a ...
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cancer research     Volume:  48     ISSN:  0008-5472     ISO Abbreviation:  Cancer Res.     Publication Date:  1988 Jul 
Date Detail:
Created Date:  1988-08-11     Completed Date:  1988-08-11     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  2984705R     Medline TA:  Cancer Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  3932-8     Citation Subset:  IM    
Affiliation:
Department of Radiology, University of Utah Health Sciences Center, Salt Lake City 84132.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line
Cell Survival*
Cricetinae
Cricetulus
DNA Topoisomerases, Type II / antagonists & inhibitors*
Female
Hela Cells / drug effects
Hot Temperature*
Humans
Nalidixic Acid / pharmacology
Novobiocin / pharmacology
Nucleoproteins / metabolism
Ovary / drug effects
Oxolinic Acid / pharmacology
Grant Support
ID/Acronym/Agency:
CA-25957/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Nucleoproteins; 14698-29-4/Oxolinic Acid; 303-81-1/Novobiocin; 389-08-2/Nalidixic Acid; EC 5.99.1.3/DNA Topoisomerases, Type II

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Inhibitory effects of 12-O-tetradecanoylphorbol-13-acetate and teleocidin B induced Epstein-Barr vir...
Next Document:  Induction of in vitro tumoricidal activity in alveolar macrophages and monocytes from patients with ...