Document Detail


Effect of time of extraction on the biocompatibility of endodontic sealers with primary human fibroblasts.
MedLine Citation:
PMID:  23018229     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
The aim of this work was to evaluate the effects of different times of extraction on the cytotoxicity of six representatives of different root canal sealer groups-Real Seal SE, AH Plus, GuttaFlow, Sealapex, Roth 801, and ThermaSeal Plus-with human gingival fibroblasts. The materials were prepared according to manufacturers' specifications, and were incubated in culture medium (DMEM) at 37ºC for 1, 7, 14, 21, and 28 days, with daily washing, to simulate periodontal ligament clearance. Human fibroblasts were exposed to the final extracts at 24 hours, and cell viability was determined by MTT assay, with exposure to unconditioned DMEM as a negative control. Statistical analysis comparing cytotoxicities at each exposure time was performed by ANOVA with Scheffé adjustment for multiple comparisons at a 95% confidence level. Results indicated that GuttaFlow was significantly less cytotoxic than all other sealers (p < 0.05) at 1 day of extraction. After 7 days of extraction, cell viability for GuttaFlow was significantly increased as compared with that of all groups except sealer AH Plus. At day 14, cytotoxicity of Sealapex was significantly higher than that of all other sealers (p < 0.05). At days 21 and 28, there were no significant differences in cytotoxicity among sealer groups. All materials presented some level of cytotoxicity to fibroblasts, while GuttaFlow was the least cytotoxic sealer tested. However, the cytotoxicity of all materials seemed to decrease similarly in a time-dependent manner.
Authors:
Miriam Zaccaro Scelza; Jeffrey Coil; Gutemberg Gomes Alves
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Brazilian oral research     Volume:  26     ISSN:  1807-3107     ISO Abbreviation:  Braz Oral Res     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-09-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101307187     Medline TA:  Braz Oral Res     Country:  Brazil    
Other Details:
Languages:  eng     Pagination:  424-30     Citation Subset:  D; IM    
Affiliation:
Laboratory of Experimental Cell Culture, Department of Endodontics, Univ Federal Fluminense - UFF, Niteroi, RJ, Brazil.
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