| Effect of thyroid status on the expression of metabolic enzymes during chronic stimulation. | |
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MedLine Citation:
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PMID: 1329543 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The effect of thyroid status on the expression of cytochrome c oxidase (CYTOX) and the activities of citrate synthase (CS) and phosphofructokinase (PFK) were examined in chronically stimulated (10 Hz; 35 days) and contralateral, nonstimulated rat tibialis anterior muscle of hypothyroid, hyperthyroid, and euthyroid animals. Stimulation increased CYTOX activity by 2.7-, 3.2-, and 4.9-fold in hyperthyroid, euthyroid, and hypothyroid animals, respectively, to similar absolute values. CS displayed similar increases. Stimulation reduced PFK activity in hypothyroid and euthyroid animals to 45% and 60% of control values. This effect was abolished with hyperthyroidism. Thus stimulation and thyroid hormone act antagonistically on PFK activity. Stimulation increased CYTOX subunit III (mitochondrially encoded) mRNA by 2.5- and 2.9-fold in hyperthyroid and euthyroid animals. Similar increases were observed in the nuclear-encoded mRNAs of CYTOX subunit VIc in euthyroid muscle. In hyperthyroid and euthyroid conditions, the mRNA changes paralleled the increases in enzyme activity. In hypothyroid muscle, the increase in mRNA was less for subunit VIc than III, suggesting that hypothyroidism upsets the coordinate expression of nuclear and mitochondrial genes. Further, the increases in CYTOX activity exceeded that of both subunit mRNAs in hypothyroid muscle.(ABSTRACT TRUNCATED AT 250 WORDS) |
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Authors:
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D A Hood; J A Simoneau; A M Kelly; D Pette |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The American journal of physiology Volume: 263 ISSN: 0002-9513 ISO Abbreviation: Am. J. Physiol. Publication Date: 1992 Oct |
Date Detail:
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Created Date: 1992-11-25 Completed Date: 1992-11-25 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0370511 Medline TA: Am J Physiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: C788-93 Citation Subset: IM |
Affiliation:
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Fakultät für Biologie, Universität Konstanz, Federal Republic of Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Citrate (si)-Synthase / metabolism* Electric Stimulation Electron Transport Complex IV / chemistry, genetics, metabolism* Hyperthyroidism / metabolism Hypothyroidism / metabolism Male Muscles / metabolism Phosphofructokinase-1 / metabolism* RNA, Messenger / metabolism Rats Rats, Wistar Reference Values Thyroid Gland / enzymology, physiology* Time Factors |
| Chemical | |
Reg. No./Substance:
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0/RNA, Messenger; EC 1.9.3.1/Electron Transport Complex IV; EC 2.3.3.1/Citrate (si)-Synthase; EC 2.7.1.11/Phosphofructokinase-1 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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