Document Detail

Effect of tetracosahexaenoic acid on the content and release of histamine, and eicosanoid production in MC/9 mouse mast cell.
MedLine Citation:
PMID:  9870906     Owner:  NLM     Status:  MEDLINE    
6,9,12,15,18,21-Tetracosahexaenoic acid (24:6n-3) was isolated from a brittle star, Ophiura sarsi Lütken, at >95% purity to evaluate its physiological functions. The effects of 24:6n-3 on the production of leukotriene (LT)-related compounds such as LTB4, LTC4 and 5-hydroxyeicosatetraenoic acid, and the accumulation and release of histamine in an MC/9 mouse mast cell line were studied. We found that 24:6n-3 could inhibit the antigen-stimulated production of LT-related compounds as well as other n-3 polyunsaturated fatty acids (PUFA) such as eicosapentaenoic acid (20:5n-3) and docosahexaenoic acid (22:6n-3), which are major n-3 PUFA in fish oils; 24:6n-3 was also shown to reduce the histamine content in MC/9 cells at 25 microM (27% reduction from the control), and the effect was diminished with increase of the fatty acid concentration (up to 100 microM). These two n-3 PUFA, 20:5n-3 and 22:6n-3, also reduced the histamine content (16 and 20% reduction at 25 microM, respectively), whereas arachidonic acid (20:4n-6) increased it (18% increase at 25 microM). Spontaneous- and antigen-induced release of histamine was not influenced with these PUFA (at 25 microM). Ionophore-stimulated release of histamine was suppressed by the PUFA (13, 9, 15, and 11% reduction with 20:4n-6, 20:5n-3, 22:6n-3, and 24:6n-3, respectively). The patterns of the effects of 24:6n-3 on the synthesis of eicosanoids and histamine content were more similar to those of 22:6n-3 than 20:5n-3. From these results, 24:6n-3 can be expected to have anti-inflammatory activity and antiallergic activities similar to those of 22:6n-3.
K Ishihara; M Murata; M Kaneniwa; H Saito; K Shinohara; M Maeda-Yamamoto; K Kawasaki; T Ooizumi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Lipids     Volume:  33     ISSN:  0024-4201     ISO Abbreviation:  Lipids     Publication Date:  1998 Nov 
Date Detail:
Created Date:  1999-03-18     Completed Date:  1999-03-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0060450     Medline TA:  Lipids     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1107-14     Citation Subset:  IM    
National Research Institute of Fisheries Science, MAFF, Yokohama, Japan.
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MeSH Terms
Antigens / pharmacology
Cell Line
Docosahexaenoic Acids / isolation & purification,  pharmacology*
Dose-Response Relationship, Drug
Echinodermata / chemistry
Eicosanoids / biosynthesis*
Fatty Acids, Omega-3 / pharmacology
Histamine Release / drug effects*
Ionophores / pharmacology
Mast Cells / cytology,  drug effects*,  metabolism
Reg. No./Substance:
0/Antigens; 0/Eicosanoids; 0/Fatty Acids, Omega-3; 0/Ionophores; 25167-62-8/Docosahexaenoic Acids; 81247-23-6/tetracosahexaenoic acid

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