Document Detail

Effect of temperature and/or pressure on lactoperoxidase activity in bovine milk and acid whey.
MedLine Citation:
PMID:  11928959     Owner:  NLM     Status:  MEDLINE    
At atmospheric pressure, inactivation of lactoperoxidase (LPO) in milk and whey was studied in a temperature range of 69-73 degrees C and followed first order kinetics. Temperature dependence of the first order inactivation rate constants could be accurately described by the Arrhenius equation, with an activation energy of 635.3 +/- 70.7 kJ/mol for raw bovine milk and 736.9 +/- 40.9 kJ/mol for diluted whey, indicating a very high temperature sensitivity. On the other hand, LPO is very pressure resistant and not or only slightly affected by treatment at pressure up to 700 MPa combined with temperatures between 20 and 65 degrees C. Both for thermal and pressure treatment, stability of LPO was higher in milk than in diluted whey. Besides, a very pronounced antagonistic effect between high temperature and pressure was observed, i.e. at 73 degrees C, a temperature where thermal inactivation at atmospheric pressure occurs rapidly, application of pressure up to 700 MPa exerted a protective effect. At atmospheric pressure, LPO in diluted whey was optimally active at a temperature of about 50 degrees C. At all temperatures studied (20-60 degrees C), LPO remained active during pressure treatment up to 300 MPa, although the activity was significantly reduced at pressures higher than 100 MPa. The optimal temperature was found to shift to lower values (30-40 degrees C) with increasing pressure.
L R Ludikhuyze; W L Claeys; M E Hendrickx
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of dairy research     Volume:  68     ISSN:  0022-0299     ISO Abbreviation:  J. Dairy Res.     Publication Date:  2001 Nov 
Date Detail:
Created Date:  2002-04-03     Completed Date:  2002-08-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985125R     Medline TA:  J Dairy Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  625-37     Citation Subset:  IM    
Department of Food and Microbial Technology, Faculty of Agricultural and Applied Biological Sciences, Katholieke Universiteit Leuven, Heverlee, Belgium.
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MeSH Terms
Enzyme Activation
Enzyme Stability
Lactoperoxidase / metabolism*
Milk / enzymology*
Milk Proteins / metabolism*
Reg. No./Substance:
0/Milk Proteins; 0/whey protein; EC 1.11.1.-/Lactoperoxidase

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