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Effect of tanshinone II A on TLR4 and TNF-α of endothelial cells induced by LPS.
MedLine Citation:
PMID:  21722522     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
AIM: To observe the influence of tanshinone II A on the expression of toll like receptor 4(TLR4) and tumor necrosis factor-α(TNF-α) in cultured human umbilical veins endothelial cells EA.hy926 induced by LPS, and to investigate the molecular mechanism of tanshinone II A against atherosclerosis(AS). METHODS: The EA.hy926 cells were cultured in vitro. The mRNA and protein expression of TLR4 were tested by RT-PCR and immunocytochemistry technique respectively. The mRNA and protein expression of TNF-α were detected by RT-PCR and ELISA technique respectively. RESULTS: The expression of TLR4 and TNF-α were both increased compared to normal group after stimulation of LPS(P<0.05). The expression of TLR4 and TNF-α in tanshinone II A group were obviously inhibited compared to stimulation group(P<0.05). CONCLUSION: The molecular mechanism of tanshinone II A against AS is probably to inhibit the expression of TLR4 and TNF-α.
Authors:
Lian-Qun Jia; Jun-Yi Feng; Guan-Lin Yang; Wen-Na Chen; Yang Chen
Publication Detail:
Type:  JOURNAL ARTICLE    
Journal Detail:
Title:  Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology     Volume:  27     ISSN:  1007-8738     ISO Abbreviation:  -     Publication Date:  2011 Jul 
Date Detail:
Created Date:  2011-7-4     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101139110     Medline TA:  Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi     Country:  -    
Other Details:
Languages:  CHI     Pagination:  733-735     Citation Subset:  -    
Affiliation:
Basic Medical College, Liaoning University of Traditional Chinese Medicine, Affliated Hospital of Liaoning University of Traditional Chinese Medicine Shenyang 110032, China.
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