Document Detail


Effect of synovial membrane infection in vitro on equine synoviocytes and chondrocytes.
MedLine Citation:
PMID:  9522948     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: To determine the functional response of synovium to infection, and the influence of infected synovium on articular cartilage metabolism. SAMPLE POPULATION: Synovium and articular cartilage explants from the midcarpal and tarsocrural joints of adult horses. PROCEDURE: For experiment 1, synovium explants were incubated as follows: control--incubation in standard medium, infected (I)--incubation with Staphylococcus aureus, and infected-filtered (IF)--incubation with medium collected from the infected group and filtered (0.22-micron filter). Daily collected medium was assayed for interleukin 1 beta (IL-1 beta), IL-6, tumor necrosis factor, and hyaluronan (HA) concentrations. For experiment 2, cartilage explants were incubated as follows: control--incubation in standard medium, and IF--incubation in medium collected from infected synovium cultures and filtered. After 48 hours, explant proteoglycan synthesis and endogenous proteoglycan and glycosaminoglycan contents were determined. RESULTS: IL-1 beta and IL-6 values were significantly increased in synovium explants from the I and IF groups. Hyaluronan concentration was lower in I and IF groups. Proteoglycan synthesis and content, and total glycosaminoglycan and chondroitin sulfate concentrations, were significantly decreased in cartilage from the IF group. CONCLUSIONS: Bacterial infection was associated with decreased HA concentration and increased mediator release. These effects were also observed despite elimination of bacteria. Exposure to sterile but previously infected medium decreased articular cartilage matrix synthesis and composition. CLINICAL RELEVANCE: Resident synovial cells may contribute appreciably to articular damage during bacterial infection in the absence of migrant inflammatory cells. This response is prolonged despite elimination of the bacteria.
Authors:
J Hardy; A L Bertone; C J Malemud
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  American journal of veterinary research     Volume:  59     ISSN:  0002-9645     ISO Abbreviation:  Am. J. Vet. Res.     Publication Date:  1998 Mar 
Date Detail:
Created Date:  1998-05-08     Completed Date:  1998-05-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0375011     Medline TA:  Am J Vet Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  293-9     Citation Subset:  IM    
Affiliation:
Department of Veterinary Clinical Sciences, Ohio State University, Columbus 43210, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Arthritis, Infectious / pathology,  physiopathology,  veterinary*
Cartilage, Articular / microbiology,  pathology,  physiopathology*
Chondroitin Sulfates / metabolism
Glycosaminoglycans / metabolism
Horse Diseases*
Horses
Hyaluronic Acid / metabolism
Interleukin-1 / biosynthesis
Interleukin-6 / biosynthesis
Organ Culture Techniques
Proteoglycans / biosynthesis
Staphylococcal Infections / pathology,  physiopathology,  veterinary*
Staphylococcus aureus*
Synovial Membrane / microbiology,  pathology,  physiopathology*
Chemical
Reg. No./Substance:
0/Glycosaminoglycans; 0/Interleukin-1; 0/Interleukin-6; 0/Proteoglycans; 9004-61-9/Hyaluronic Acid; 9007-28-7/Chondroitin Sulfates

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