| Effect of surfactant on the production of oxygenated unsaturated fatty acids by Bacillus megaterium ALA2. | |
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MedLine Citation:
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PMID: 19748603 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Bacillus megaterium ALA2 NRRL B-21660 has been well studied for the production of many oxygenated unsaturated fatty acids from linoleic acid. Its major product, 12,13,17-trihydroxy-9(Z)-octadecenoic acid (12,13,17-THOA), inhibited the growth of many plant pathogenic fungi. However, we have been unable, until now, to demonstrate 12,13,17-THOA production in a fermentor. Here, we have investigated the effect of surfactants on 12,13,17-THOA production. Surfactant types (SO-25, Tween-80 and Triton X-100) at various concentrations were evaluated for their effects on cell growth and production of 12,13,17-THOA. Triton X-100 decreased cell growth and 12,13,17-THOA production while 1% Tween-80 increased 12,13,17-THOA production more than twofold over control. In a pH and dissolved oxygen (DO) controlled fermentor and with 1.0% Tween-80, both 12,13,17-THOA production and productivity increased to 553 mg/L and 24.0mg/L/hour, respectively. We further conducted THOA production in a fermentor without the control of pH and DO, but with aeration through medium surface rather than by Spurger, and with various amounts of Tween-80 to compare with the results obtained in flask runs. Both additions with 0.1% and 0.5% Tween-80 and the control runs produced poor amounts of product 12,13,17-THOA. The maximum 12,13,17-THOA production was observed at 1.0% Tween-80 at a yield of 650 mg/L. Thus fermentor production of 12,13,17-THOA was successfully demonstrated. |
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Authors:
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Beom Soo Kim; Hak-Ryul Kim; Ching T Hou |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-09-11 |
Journal Detail:
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Title: New biotechnology Volume: 27 ISSN: 1876-4347 ISO Abbreviation: N Biotechnol Publication Date: 2010 Feb |
Date Detail:
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Created Date: 2010-03-01 Completed Date: 2010-05-24 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101465345 Medline TA: N Biotechnol Country: Netherlands |
Other Details:
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Languages: eng Pagination: 33-7 Citation Subset: IM |
Copyright Information:
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Published by Elsevier B.V. |
Affiliation:
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Microbial Genomics and Bioprocessing Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, United States Department of Agriculture, Peoria, IL 61604, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bacillus megaterium
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metabolism* Fatty Acids, Unsaturated / biosynthesis*, chemistry Fermentation Linoleic Acid / metabolism Oxygen / metabolism* Soybean Oil / chemistry Surface-Active Agents / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids, Unsaturated; 0/Surface-Active Agents; 2197-37-7/Linoleic Acid; 7782-44-7/Oxygen; 8001-22-7/Soybean Oil |
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