| Effect of surface treatment on cell responses to grades 4 and 5 titanium for orthodontic mini-implants. | |
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MedLine Citation:
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PMID: 22640672 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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INTRODUCTION: Mini-implants are used to improve orthodontic anchorage, but optimal composition and surface characteristics have yet to be determined. We investigated the behavior of osteoblast-like cells on grade 4 commercially pure titanium and grade 5 titanium alloy with different surface treatments for mini-implants. METHODS: MC3T3 cells were plated on machined, acid-etched, or acid-etched grade 4 titanium enriched with calcium phosphate, or machined, anodized, or anodized and calcium phosphate-enriched grade 5 titanium disks. Surface and cell morphologies were assessed by scanning electron microscopy. Cell viability was measured by chemiluminescence, cytoskeletal organization was investigated by immunofluorescence, and real-time polymerase chain reaction for osteoblast-specific genes was performed to measure cell differentiation. RESULTS: Flattened shapes and strong stress fibers were observed on the machined surfaces; cells on the rough surfaces had a spindle shape, with lower cytoskeletal polarization. Cell proliferation was highest on smooth grade 4 titanium surfaces, whereas cells quickly reached a plateau on rough grade 4 titanium; no difference was observed after 72 hours in the grade 5 titanium groups. Calcium phosphate enrichment on grade 4 titanium significantly increased the messenger RNA levels for alkaline phosphatase and osteocalcin. Osteoblastic markers were higher on the grade 5 titanium machined surfaces than on the rough surfaces, and comparable with acid-etched grade 4 titanium. CONCLUSIONS: Although the grade 4 titanium enriched with calcium phosphate had the highest level of differentiation in vitro, the grade 5 titanium machined surfaces supported cell proliferation and matrix synthesis, and induced high expression of early differentiation markers. Increased mechanical resistance of grade 5 titanium makes it a potential candidate for orthodontic mini-implants. |
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Authors:
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Carlo Galli; Marilina Piemontese; Francesca Ravanetti; Simone Lumetti; Giovanni Passeri; Mauro Gandolfini; Guido M Macaluso |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: American journal of orthodontics and dentofacial orthopedics : official publication of the American Association of Orthodontists, its constituent societies, and the American Board of Orthodontics Volume: 141 ISSN: 1097-6752 ISO Abbreviation: Am J Orthod Dentofacial Orthop Publication Date: 2012 Jun |
Date Detail:
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Created Date: 2012-05-29 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8610224 Medline TA: Am J Orthod Dentofacial Orthop Country: United States |
Other Details:
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Languages: eng Pagination: 705-14 Citation Subset: D; IM |
Copyright Information:
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Copyright © 2012 American Association of Orthodontists. Published by Mosby, Inc. All rights reserved. |
Affiliation:
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Research associate, Section of Odontostomatologia, University of Parma, Parma, Italy. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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