Document Detail

Effect of sulfhydryl-binding reagents on release of somatostatin immunoreactivity from secretion granules.
MedLine Citation:
PMID:  6757085     Owner:  NLM     Status:  MEDLINE    
The effect of various sulfhydryl-binding reagents on the release of somatostatin immunoreactivity (SRIF-I) from secretion granules of the islets of Langerhans was studied. The granules were isolated from toadfish islet tissue by differential centrifugation in sucrose. They were then incubated in the test medium, pelleted, and the supernatant was assayed for SRIF-I using a radioimmunoassay. When the granules were incubated in isotonic saline, about 20% of the total SRIF-I in the granules was released into the medium. Three mercurials (p-hydroxymercuribenzoate, phenylmercuric acetate and Hg++) and an arsenical (diphenylchloroarsine) increased SRIF-I release 1.5 to 2-fold. In contrast, other heavy metals (Co++ and Cd++), other arsenicals (dichlorophenylarsine and AsO-2), alkylating agents (N-ethylmaleimide and iodoacetic acid), an oxidizing agent (oxidized glutathione), a reducing agent (reduced glutathione), and an amino group-binding reagent (trinitrobenzene sulfonic acid), had no effect. These results suggest that sulfhydryl groups may be present on the somatostatin secretion granules and, if so, that they differ from those previously demonstrated on the insulin- and glucagon-secretion granules.
T F Winters; S J Cooperstein; D T Watkins
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et métabolisme     Volume:  14     ISSN:  0018-5043     ISO Abbreviation:  Horm. Metab. Res.     Publication Date:  1982 Oct 
Date Detail:
Created Date:  1983-02-14     Completed Date:  1983-02-14     Revised Date:  2009-02-19    
Medline Journal Info:
Nlm Unique ID:  0177722     Medline TA:  Horm Metab Res     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  525-9     Citation Subset:  IM    
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MeSH Terms
Cadmium / pharmacology
Cobalt / pharmacology
Cytoplasmic Granules / drug effects,  metabolism*
Ethylmaleimide / pharmacology
Glutathione / pharmacology
Iodoacetates / pharmacology
Iodoacetic Acid
Islets of Langerhans / ultrastructure
Peptides / metabolism*
Sulfhydryl Reagents / pharmacology*
Grant Support
Reg. No./Substance:
0/Iodoacetates; 0/Peptides; 0/Sulfhydryl Reagents; 0/somatostatin-like peptides; 128-53-0/Ethylmaleimide; 64-69-7/Iodoacetic Acid; 70-18-8/Glutathione; 7440-43-9/Cadmium; 7440-48-4/Cobalt

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