| Effect of storage temperature during transport of ovaries on in vitro embryo production in Iberian red deer (Cervus elaphus hispanicus). | |
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MedLine Citation:
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PMID: 21040958 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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The aim of this work was to study the effect of storage temperature during the transport of ovaries on cleavage and blastocyst rates in Iberian red deer, because wild populations of this subspecies are usually far from laboratories. A total of 472 ovaries from 236 Iberian hinds were recovered and maintained in saline solution at 5-8 °C or 20-25 °C for 12 h. After storage, aspirated oocytes were matured with FSH/LH or EGF and the developed embryos were cultured with oviduct epithelial cells monolayer (OCM). A higher (P = 0.009) cleavage rate was obtained when the ovaries were stored at 5-8 °C. However, there were no differences between both storage temperatures in relation to the percentage of blastocysts obtained. Considering the management and production systems of Iberian red deer, this study provides important information about the ovary storage temperature during transport with the purpose of assuring an optimal in vitro embryo production. |
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Authors:
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O García-Alvarez; A Maroto-Morales; F Berlinguer; M R Fernández-Santos; M C Esteso; P Mermillod; J A Ortiz; M Ramon; M D Pérez-Guzmán; J J Garde; A J Soler |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-10-30 |
Journal Detail:
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Title: Theriogenology Volume: 75 ISSN: 1879-3231 ISO Abbreviation: Theriogenology Publication Date: 2011 Jan |
Date Detail:
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Created Date: 2010-12-07 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0421510 Medline TA: Theriogenology Country: United States |
Other Details:
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Languages: eng Pagination: 65-72 Citation Subset: IM |
Copyright Information:
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Copyright © 2011 Elsevier Inc. All rights reserved. |
Affiliation:
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Regional Center of Animal Selection and Reproduction (CERSYRA) JCCM, Valdepeñas, Spain. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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