Document Detail


Effect of storage in juice with or without pulp and/or calcium lactate on the subsequent survival of Escherichia coli O157:H7 in simulated gastric fluid.
MedLine Citation:
PMID:  18328587     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A study was conducted to evaluate the effect of storing Escherichia coli O157:H7 in fruit or vegetable juices with or without pulp and/or calcium lactate, on the bacterial resistance to a simulated gastric fluid (SGF, pH 1.5). Apple, carrot, orange, and tomato juices containing pulp or freed from pulp by filtration were used in this study. Calcium lactate at about 1.4 g/l was added to juices to obtain calcium supplemented juices. Juices with or without pulp and/or calcium lactate were inoculated with E. coli O157:H7 and then were stored at 7 degrees C for 0, 1, 2, or 4 days. The acid resistance of cells stored in juices with or without pulp and/or calcium lactate was determined by incubating in SGF for 90 or 240 min at 37 degrees C. Cells stored in apple juice for 4 days, carrot juice for 2 days, and orange juice for 4 days with pulp only had greater acid resistance, while all cells stored in tomato juice with pulp had greater acid resistance than cells stored in juice without pulp. The D-values of cells stored in supplemented apple and orange juices with calcium lactate declined 1.7-3.5 fold, whereas D-values of cells stored in supplemented tomato juice decreased by about 1.4-fold when compared to cells stored in juice without calcium lactate after exposure in SGF. These results indicate that storing E. coli O157:H7 in juices with pulp had little or no effect on the acid resistance of cells during subsequent exposure in SGF. Calcium lactate supplemented into juices could dramatically decrease the ability of E. coli O157:H7 to survive in SGF, possibly reducing the risk of foodborne illness by juice products.
Authors:
Hyun-Gyun Yuk; Seong-Chun Jo; Hye-Kyung Seo; Sun-Min Park; Seung-Cheol Lee
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-02-05
Journal Detail:
Title:  International journal of food microbiology     Volume:  123     ISSN:  0168-1605     ISO Abbreviation:  Int. J. Food Microbiol.     Publication Date:  2008 Apr 
Date Detail:
Created Date:  2008-04-29     Completed Date:  2008-08-04     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8412849     Medline TA:  Int J Food Microbiol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  198-203     Citation Subset:  IM    
Affiliation:
Department of Food Science and Human Nutrition, University of Florida, Gainesville, FL 32611-0370, USA.
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MeSH Terms
Descriptor/Qualifier:
Beverages / microbiology*
Calcium Compounds / pharmacology*
Citrus / microbiology
Cold Temperature
Colony Count, Microbial
Consumer Product Safety
Daucus carota / microbiology
Escherichia coli O157 / growth & development*
Food Contamination / analysis*
Food Microbiology
Food Preservation / methods*
Humans
Hydrochloric Acid / pharmacology*
Hydrogen-Ion Concentration
Lactates / pharmacology*
Lycopersicon esculentum / microbiology
Malus / microbiology
Time Factors
Chemical
Reg. No./Substance:
0/Calcium Compounds; 0/Lactates; 0/calcium lactate; 7647-01-0/Hydrochloric Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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