Document Detail

Effect of storage duration on the mechanical behavior of mouse carotid artery.
MedLine Citation:
PMID:  21823746     Owner:  NLM     Status:  MEDLINE    
Determining arterial mechanical properties is important for understanding the work done by the heart and how it changes with cardiovascular disease. Ex vivo tests are necessary to apply various loads to the artery and obtain data to model and predict the behavior under any load. Most ex vivo tests are performed within 24 h of dissection, so the tissue is still "alive." For large elastic arteries; however, the passive mechanical behavior is attributed mostly to the very stable proteins, elastin, and collagen. If the testing equipment fails, is in use, or is located at another facility, it would be useful to store the vessels and postpone the tests until the equipment is available. The goal of this study is to determine the effects of storage time on the mechanical behavior of the common carotid artery from adult mice. Each artery was tested after storage for 1-28 days in physiologic saline at 4°C. There were no significant effects of storage time on the arterial diameter or force at each pressure, but there were significant effects on the stretch ratio and stress at each pressure. The significant effects on the stretch ratio and stress were due to decreases in the unloaded dimensions with storage time, when measured from cut arterial rings. When the unloaded dimensions were measured instead from histology sections, there were no significant changes with storage time. We conclude that histology sections yield a more consistent measurement of the unloaded dimensions and that there are no significant changes in the mechanical behavior of mouse carotid artery with storage up to 28 days.
Mazyar Amin; Amber G Kunkel; Victoria P Le; Jessica E Wagenseil
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Journal of biomechanical engineering     Volume:  133     ISSN:  1528-8951     ISO Abbreviation:  J Biomech Eng     Publication Date:  2011 Jul 
Date Detail:
Created Date:  2011-08-09     Completed Date:  2011-11-30     Revised Date:  2013-06-28    
Medline Journal Info:
Nlm Unique ID:  7909584     Medline TA:  J Biomech Eng     Country:  United States    
Other Details:
Languages:  eng     Pagination:  071007     Citation Subset:  IM    
Department of Biomedical Engineering, St. Louis University, St. Louis, MO 63103, USA.
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MeSH Terms
Carotid Artery, Common / physiology*
Collagen / metabolism
Computer Simulation
Dissection / methods
Elastin / metabolism
Mice, Inbred C57BL
Models, Cardiovascular*
Stress, Mechanical*
Time Factors
Tissue Preservation / methods*
Grant Support
Reg. No./Substance:
9007-34-5/Collagen; 9007-58-3/Elastin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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