Document Detail

Effect of static pressure on intracellular pH of adhesive Chinese hamster ovary cells.
MedLine Citation:
PMID:  18215639     Owner:  NLM     Status:  MEDLINE    
The effect of static pressure on the intracellular pH of the Chinese hamster ovary (CHO) cell line DR1000L4N was investigated. In cultivation of CHO cells at 0.9 MPa, two distinct populations were observed in the histogram of a flow cytometer, while single population was observed in cultivation at 0.1 MPa. The intracellular pH of the major population at 0.9 MPa was markedly lower than that of the single population at 0.1 MPa.
Masashi Fujiwara; Satoru Koizumi; Mutsumi Takagi
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of bioscience and bioengineering     Volume:  104     ISSN:  1389-1723     ISO Abbreviation:  J. Biosci. Bioeng.     Publication Date:  2007 Dec 
Date Detail:
Created Date:  2008-01-24     Completed Date:  2008-02-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100888800     Medline TA:  J Biosci Bioeng     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  510-2     Citation Subset:  IM    
Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, Kita 13, Nishi 8, Kita-ku, Sapporo 060-8628, Japan.
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MeSH Terms
CHO Cells / chemistry*,  physiology*
Cell Adhesion / physiology
Computer Simulation
Hydrogen-Ion Concentration
Mechanotransduction, Cellular / physiology*
Models, Biological*

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