Document Detail


Effect of sonication and serum proteins on copper release from copper nanoparticles and the toxicity towards lung epithelial cells.
MedLine Citation:
PMID:  21117831     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Abstract Different methodological settings can influence particle characteristics and toxicity in nanotoxicology. The aim of this study was to investigate how serum proteins and sonication of Cu nanoparticle suspensions influence the properties of the nanoparticles and toxicological responses on human lung epithelial cells. This was investigated by using methods for particle characterization (photon correlation spectroscopy and TEM) and Cu release (atomic absorption spectroscopy) in combination with assays for analyzing cell toxicity (MTT-, trypan blue- and Comet assay). The results showed that sonication of Cu nanoparticles caused decreased cell viability and increased Cu release compared to non-sonicated particles. Furthermore, serum in the cell medium resulted in less particle agglomeration and increased Cu release compared with medium without serum, but no clear difference in toxicity was detected. Few cells showed intracellular Cu nanoparticles due to fast release/dissolution processes of Cu. In conclusion; sonication can affect the toxicity of nanoparticles.
Authors:
Pontus Cronholm; Klara Midander; Hanna L Karlsson; Karine Elihn; Inger Odnevall Wallinder; Lennart Möller
Publication Detail:
Type:  Journal Article     Date:  2010-11-30
Journal Detail:
Title:  Nanotoxicology     Volume:  5     ISSN:  1743-5404     ISO Abbreviation:  Nanotoxicology     Publication Date:  2011 Jun 
Date Detail:
Created Date:  2011-05-25     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101233132     Medline TA:  Nanotoxicology     Country:  England    
Other Details:
Languages:  eng     Pagination:  269-81     Citation Subset:  IM    
Affiliation:
Unit for Analytical Toxicology, Department of Biosciences and Nutrition at Novum, Karolinska Institutet , Stockholm , Sweden.
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