Document Detail


Effect of solvent on cytotoxicity and bioavailability of fatty acids.
MedLine Citation:
PMID:  20095809     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Fatty acids (FAs) represent an important part of cell membranes and a source of energy. However, their abundance is linked with several diseases such as type 2 diabetes mellitus, and for this reason they are studied intensively. In this article we compare the two main methods of dissolving FAs for work in vitro, (i) dissolution in dimethylsulfoxide (DMSO) and (ii) conjugation with bovine serum albumin (BSA), and describe the effects of the solvent on cytotoxicity (determination of viability) and bioavailability (as shown by the impact on the gene expression of TNF-alpha). We have found that conjugation with BSA is significantly less cytotoxic than dissolution in DMSO and also yields greater bioavailability.
Authors:
Jan Hosek; Veronika Zavalova; Peter Kollar
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Immunopharmacology and immunotoxicology     Volume:  32     ISSN:  1532-2513     ISO Abbreviation:  Immunopharmacol Immunotoxicol     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-08-09     Completed Date:  2010-12-07     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8800150     Medline TA:  Immunopharmacol Immunotoxicol     Country:  England    
Other Details:
Languages:  eng     Pagination:  462-5     Citation Subset:  IM    
Affiliation:
Department of Natural Drugs, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic. hosekj@vfu.cz
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MeSH Terms
Descriptor/Qualifier:
Biological Availability
Dimethyl Sulfoxide / chemistry*
Gene Expression / drug effects
Humans
Monocytes / drug effects
Myristic Acid / chemistry,  pharmacokinetics*,  toxicity*
Serum Albumin, Bovine / chemistry*
Solubility
Solvents / chemistry*
Tumor Cells, Cultured
Tumor Necrosis Factor-alpha / analysis
Chemical
Reg. No./Substance:
0/Serum Albumin, Bovine; 0/Solvents; 0/Tumor Necrosis Factor-alpha; 544-63-8/Myristic Acid; 67-68-5/Dimethyl Sulfoxide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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