| Effect of solvent on cytotoxicity and bioavailability of fatty acids. | |
| | |
MedLine Citation:
|
PMID: 20095809 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Fatty acids (FAs) represent an important part of cell membranes and a source of energy. However, their abundance is linked with several diseases such as type 2 diabetes mellitus, and for this reason they are studied intensively. In this article we compare the two main methods of dissolving FAs for work in vitro, (i) dissolution in dimethylsulfoxide (DMSO) and (ii) conjugation with bovine serum albumin (BSA), and describe the effects of the solvent on cytotoxicity (determination of viability) and bioavailability (as shown by the impact on the gene expression of TNF-alpha). We have found that conjugation with BSA is significantly less cytotoxic than dissolution in DMSO and also yields greater bioavailability. |
| | |
Authors:
|
Jan Hosek; Veronika Zavalova; Peter Kollar |
Related Documents
:
|
8944749 - Covalent complexes between serum albumin and 7-hydroxycoumarin-4-acetic acid: synthesis... 3185569 - Iga-mediated clearance and tissue deposition of dinitrophenylated human serum albumin a... 20211579 - Ultrasensitive determination of serum albumin using resonance light scattering based on... 2592299 - Variable responsiveness of rat tracheal epithelial cells to bovine serum albumin in ser... 1619999 - Effect of fatty acids on rat liver nuclear t3-receptor binding. 8340929 - Fatty acid transfer across the myocardial capillary wall. 11502269 - Isolation of oligogalacturonic acids up to dp 20 by preparative high-performance anion-... 2254029 - Inhibition of heat-labile toxin from bordetella parapertussis by fatty acids. 18793169 - Differential regulation of the er stress response by long-chain fatty acids in the panc... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Immunopharmacology and immunotoxicology Volume: 32 ISSN: 1532-2513 ISO Abbreviation: Immunopharmacol Immunotoxicol Publication Date: 2010 Sep |
Date Detail:
|
Created Date: 2010-08-09 Completed Date: 2010-12-07 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8800150 Medline TA: Immunopharmacol Immunotoxicol Country: England |
Other Details:
|
Languages: eng Pagination: 462-5 Citation Subset: IM |
Affiliation:
|
Department of Natural Drugs, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic. hosekj@vfu.cz |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Biological Availability Dimethyl Sulfoxide / chemistry* Gene Expression / drug effects Humans Monocytes / drug effects Myristic Acid / chemistry, pharmacokinetics*, toxicity* Serum Albumin, Bovine / chemistry* Solubility Solvents / chemistry* Tumor Cells, Cultured Tumor Necrosis Factor-alpha / analysis |
| Chemical | |
Reg. No./Substance:
|
0/Serum Albumin, Bovine; 0/Solvents; 0/Tumor Necrosis Factor-alpha; 544-63-8/Myristic Acid; 67-68-5/Dimethyl Sulfoxide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: The effect of MS14 on Th2 cytokines pattern in Balb/C mice.
Next Document: Exploring the potential of Nelumbo nucifera rhizome on membrane stabilization, mast cell protection,...