Document Detail


Effect of soil clay content on RNA isolation and on detection and quantification of bacterial gene transcripts in soil by quantitative reverse transcription-PCR.
MedLine Citation:
PMID:  21724880     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this study, we evaluated the effect of soil clay content on RNA isolation and on quantitative reverse transcription-PCR (qRT-PCR) quantification of microbial gene transcripts. The amount of clay significantly altered RNA isolation yields and qRT-PCR analyses. Recommendations are made for quantifying microbial gene transcripts in soil samples varying in clay content.
Authors:
A Novinscak; M Filion
Publication Detail:
Type:  Journal Article     Date:  2011-07-01
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  77     ISSN:  1098-5336     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2011 Sep 
Date Detail:
Created Date:  2011-08-25     Completed Date:  2011-12-13     Revised Date:  2013-06-28    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  6249-52     Citation Subset:  IM    
Affiliation:
Université de Moncton, Biology Department, Moncton, New Brunswick, Canada.
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MeSH Terms
Descriptor/Qualifier:
Aluminum Silicates / analysis*
RNA, Messenger / analysis*,  isolation & purification*
Real-Time Polymerase Chain Reaction / methods*
Soil / chemistry*
Soil Microbiology*
Chemical
Reg. No./Substance:
0/Aluminum Silicates; 0/RNA, Messenger; 0/Soil; 1302-87-0/clay
Comments/Corrections

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