Document Detail


Effect of small interfering RNAs of cytochrome P450 17alpha-hydroxylase/17,20-lyase (CYP17) on androgen biosynthesis in theca cells.
MedLine Citation:
PMID:  18262808     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Polycystic ovary syndrome (PCOS) is associated with a variety of endocrinologic and metabolic abnormalities, with clinical features of hyperandrogenism and hyperandrogenemia. Cytochrome P450 17alpha-hydroxylase/17,20-lyase (CYP17) is critical in androgen biosynthesis, and CYP17 mRNA expression was proven augmented in PCOS theca cells. To demonstrate whether RNA interference (RNAi) could lower the androgen concentration in theca cells, small interfering RNA (siRNA) targeting the CYP17 gene was co-cultured with exogenous CYP17 in HeLa cells and endogenous CYP17 of theca cells. CYP17 gene expression was measured by fluorescent microscopy, flow cytometry and real-time reverse transcription-polymerase chain reaction analysis. Androstenedione and progesterone concentrations were measured by ELISA. RNAi effectively reduced the expression of exogenous CYP17 in HeLa cells by up to 50%. The CYP17 mRNA and androstenedione production of theca cells were slightly, but not significantly, reduced when compared with non-specific siRNA.
Authors:
Jing Du; Xiaoyan Liang; Haitao Zeng; Yimin Shu; Shuzhong Yao; Bo Zhu; Guanglun Zhuang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-01-10
Journal Detail:
Title:  Cell biology international     Volume:  32     ISSN:  1065-6995     ISO Abbreviation:  Cell Biol. Int.     Publication Date:  2008 Apr 
Date Detail:
Created Date:  2008-05-02     Completed Date:  2008-07-24     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9307129     Medline TA:  Cell Biol Int     Country:  England    
Other Details:
Languages:  eng     Pagination:  469-72     Citation Subset:  IM    
Affiliation:
Center for Reproductive Medicine, First Affiliated Hospital of Sun Yat-sen University, Zhongshan Er Road, Guangzhou, Guangdong, China.
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MeSH Terms
Descriptor/Qualifier:
Adult
Androgens / biosynthesis*
Female
Gene Silencing / drug effects
Hela Cells
Humans
RNA, Small Interfering / pharmacology*
Steroid 17-alpha-Hydroxylase / metabolism*
Theca Cells / drug effects,  enzymology*
Chemical
Reg. No./Substance:
0/Androgens; 0/RNA, Small Interfering; EC 1.14.99.9/Steroid 17-alpha-Hydroxylase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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