Document Detail


The effect of size and species on lens intracellular hydrostatic pressure.
MedLine Citation:
PMID:  23211824     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: Previous experiments showed that mouse lenses have an intracellular hydrostatic pressure that varied from 335 mm Hg in central fibers to 0 mm Hg in surface cells. Model calculations predicted that in larger lenses, all else equal, pressure should increase as the lens radius squared. To test this prediction, lenses of different radii from different species were studied.
METHODS: All studies were done in intact lenses. Intracellular hydrostatic pressures were measured with a microelectrode-manometer-based system. Membrane conductances were measured by frequency domain impedance analysis. Intracellular Na(+) concentrations were measured by injecting the Na(+)-sensitive dye sodium-binding benzofuran isophthalate.
RESULTS: Intracellular hydrostatic pressures were measured in lenses from mice, rats, rabbits, and dogs with radii (cm) 0.11, 0.22, 0.49, and 0.57, respectively. In each species, pressure varied from 335 ± 6 mm Hg in central fiber cells to 0 mm Hg in surface cells. Further characterization of transport in lenses from mice and rats showed that the density of fiber cell gap junction channels was approximately the same, intracellular Na(+) concentrations varied from 17 mM in central fiber cells to 7 mM in surface cells, and intracellular voltages varied from -45 mV in central fiber cells to -60 mV in surface cells. Fiber cell membrane conductance was a factor of 2.7 times larger in mouse than in rat lenses.
CONCLUSIONS: Intracellular hydrostatic pressure is an important physiological parameter that is regulated in lenses from these different species. The most likely mechanism of regulation is to reduce the density of open Na(+)-leak channels in fiber cells of larger lenses.
Authors:
Junyuan Gao; Xiurong Sun; Leon C Moore; Peter R Brink; Thomas W White; Richard T Mathias
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural     Date:  2013-01-07
Journal Detail:
Title:  Investigative ophthalmology & visual science     Volume:  54     ISSN:  1552-5783     ISO Abbreviation:  Invest. Ophthalmol. Vis. Sci.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-01-08     Completed Date:  2013-03-05     Revised Date:  2013-08-16    
Medline Journal Info:
Nlm Unique ID:  7703701     Medline TA:  Invest Ophthalmol Vis Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  183-92     Citation Subset:  IM    
Affiliation:
Department of Physiology and Biophysics, State University of New York at Stony Brook, Stony Brook, New York 11794-8661, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Benzofurans / pharmacokinetics
Dogs
Electric Impedance
Ethers, Cyclic / pharmacokinetics
Fluorescent Dyes / pharmacokinetics
Gap Junctions / physiology
Hydrostatic Pressure
Lens, Crystalline / physiology*
Manometry
Membrane Potentials / physiology
Mice
Models, Biological*
Organ Size / physiology*
Rabbits
Rats
Sodium / metabolism*
Species Specificity
Water / physiology
Grant Support
ID/Acronym/Agency:
EY06391/EY/NEI NIH HHS; EY13163/EY/NEI NIH HHS; GM088180/GM/NIGMS NIH HHS; R01 EY006391/EY/NEI NIH HHS; R01 EY013163/EY/NEI NIH HHS
Chemical
Reg. No./Substance:
0/Benzofurans; 0/Ethers, Cyclic; 0/Fluorescent Dyes; 124549-08-2/sodium-binding benzofuran isophthalate; 7440-23-5/Sodium; 7732-18-5/Water
Comments/Corrections

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